|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Osteoclasts from osteoclastomas|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||10 µl for 10^6 cells in 100 µl|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (Agglutination) (WB-Ag)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-33578 detects CD51/61 from human samples. MA1-33578 does not react with porcine, lapine or bovine samples.
MA1-33578 has been successfully used in immunoprecipitation and flow cytometry applications. For flow cytometry use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
The MA1-33578 immunogen is Osteoclasts from osteoclastomas.
MA1-33578 recognized the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Voltage-dependent anion channels (VDACs, porin) expressed in the plasma membrane regulate the differentiation and function of human osteoclasts.
MA1-33578 was used in immunocytochemistry to study voltage-dependent anion channels in human osteoclasts
|Kotake S,Yago T,Kawamoto M,Nanke Y||Cell biology international (37:65)||2013|
T-cell leukemia translocation-associated gene (TCTA) protein is required for human osteoclastogenesis.
MA1-33578 was used in immunohistochemistry to study the role of T-cell leukemia translocation-associated gene protein in human osteoclastogenesis
|Kotake S,Nanke Y,Kawamoto M,Yago T,Udagawa N,Ichikawa N,Kobashigawa T,Saito S,Momohara S,Kamatani N,Yamanaka H||Bone (45:627)||2009|
The efficiency of the translocation of Mycobacterium tuberculosis across a bilayer of epithelial and endothelial cells as a model of the alveolar wall is a consequence of transport within mononuclear phagocytes and invasion of alveolar epithelial cells.
MA1-33578 was used in blocking assay to investigate translocation of Mycobacterium tuberculosis across the lung.
|Bermudez LE,Sangari FJ,Kolonoski P,Petrofsky M,Goodman J||Infection and immunity (70:140)||2002|