Invitrogen

Ki-67 Monoclonal Antibody (SolA15), eBioscience™

Product Image Gallery

Ki-67 Antibody — Figure 1 ATF3 maintains intestinal homeostasis. (A) Comparison of colon length between naive mice as indicated. (B) Colon crypts from mice were isolated by shaking colon fragments in EDTA and counted under light microscopy. (C) Flow cytometry analysis of Ki67 and CD24 expression in ileum crypts, gated on the CD45 - EpCAM + populations, from the indicated naive mice. (D) Representative micrographs showing intestinal organoids derived from naive mice. (E) Quantitative real-time PCR analysis of cell cycle genes in naive ileum organoids at day 6 of culture (""n"" indicates organoids derived from 4 mice each group). (F) Representative confocal images of whole mount tissues with co-immunofluorescence staining of UEA-1 and WGA in naive ileum villi. Results were from at least two independent experiments and ""n"" refers to the number of mice unless indicated otherwise. All mice were at the age of 2~3 months old when analyzed. Statistical analysis was done using Multiple T -test on Prism software. * P < 0.05, ** P < 0.005, *** P < 0.0005.

Ki-67 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry on fixed and permeabilized C2C12 cells using 5 µg/mL of Rat IgG2a Isotype Control (left) or 5 µg/mL of Anti-Mouse/Rat Ki-67 Purified (right) followed by Anti-Rat TRITC. Nuclei are stained with DAPI. Co-expression of Ki-67 and DAPI appears pink.

Ki-67 Antibody in Immunohistochemistry (IHC) — Immunofluorescent analysis was performed with formalin-fixed paraffin embedded mouse stomach tumor sections. The slides were de-paraffinization and undergo antigen retrieval in TE pH9. The sections were blocked with 5% normal donkey serum in PBS for an hour, then incubated with the Rat anti-Ki67 antibody (Product # 14-5698-80) diluted 1:200 overnight. The slides were then washed three times with TBS, and incubated with the CY3 conjugated donkey anti-rat Cy3 secondary antibody for 1.5 hours. Finally the slides were counterstained with Hoechst (Product # 62249) diluted 1:5000 before mounting in Prolong Gold anti-fad mounting media (Product # P36931). Images were acquired using a confocal microscope. The positive and negative images show sections with and without the primary antibody respectively, acquired with the same imaging parameters. Data courtesy of Antibody Data Exchange Program.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5 µg/mL

Published Applications

Flow Cytometry (Flow)

See 38 publications below

Immunofluorescence (IF)

See 30 publications below

Immunocytochemistry (ICC)

See 3 publications below

Immunohistochemistry (IHC)

See 17 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 6 publications below

Western Blot (WB)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Dog, Cynomolgus Monkey, Human, Mouse, Non-human primate, Rat

Published species

Fish, Human, Mouse, Rat

Host / Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

SolA15

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10854564

Product Specific Information

Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. Ki-67 is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0). Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. Ki-67 is used as a marker for determining the growth fraction of a given population of cells. In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin).

The SolA15 antibody also recognizes human, non-human primate and canine Ki-67.

Applications Reported: This SolA15 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (FFPE) tissue sections and frozen (paraformaldehyde fixed) sections and immunocytochemistry (ICC).

Applications Tested: This SolA15 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded mouse skin with high or low pH antigen retrieval and immunocytochemistry on fixed and permeabilized C2C12 cells at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; KIA; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2

Gene Aliases: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105

UniProt ID: (Human) P46013, (Mouse) E9PVX6

Entrez Gene ID: (Dog) 100686578, (Human) 4288, (Cynomolgus Monkey) 102135895, (Rat) 291234, (Mouse) 17345

Function(s)

DNA metabolic process meiotic nuclear division cell proliferation response to organic cyclic compound hyaluronan metabolic process organ regeneration cellular response to heat hepatocyte proliferation cholangiocyte proliferation

Process(es)

protein binding ATP binding protein C-terminus binding poly(A) RNA binding molecular_function