Invitrogen

Kinesin 5B Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (13) Kinesin 5B antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Kinesin 5B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin embedded of Human Kidney using Product # PA5-18063 at a concentration of 1 µg/mL. The tissue was processed by microwaved antigen retrieval with Tris/EDTA buffer pH 9 and stained with HRP.

Kinesin 5B Antibody in Western Blot (WB) — Western blot was performed using Anti-KIF5B Polyclonal Antibody, (Product # PA5-18063) and a ~120 kDa band corresponding to KIF5B was observed in the cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), HT-29 (Lane 3), and A-431 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Kinesin 5B Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with KIF5B siRNA and a steep decrease in signal intensity was observed in western blot application using Anti-KIF5B Polyclonal Antibody (Product # PA5-18063). {KD}

Product Details

PA5-18063

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-1.5 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide sequence (QPVAVRGGGGKQV) corresponding to the C-terminus amino acids of KIF5B View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_10984828

Product Specific Information

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Target Information

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Conventional kinesin heavy chain; epididymis secretory protein Li 61; kinesin 1 (110-120kD); kinesin heavy chain; Kinesin-1 heavy chain; U-KHC; Ubiquitous kinesin heavy chain; UKHC

Gene Aliases: HEL-S-61; KIF5B; KINH; KNS; KNS1; UKHC

UniProt ID: (Human) P33176

Entrez Gene ID: (Human) 3799

Molecular Function: microtubule binding motor protein