Invitrogen

Kinesin 5B Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (12) Kinesin 5B antibodies

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Datasheet

Questions & Answers

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Kinesin 5B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin embedded of Human Kidney using Product # PA5-18063 at a concentration of 1 µg/mL. The tissue was processed by microwaved antigen retrieval with Tris/EDTA buffer pH 9 and stained with HRP.

Kinesin 5B Antibody in Western Blot (WB) — Western blot was performed using Anti-KIF5B Polyclonal Antibody, (Product # PA5-18063) and a ~120 kDa band corresponding to KIF5B was observed in the cell lines tested. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), HT-29 (Lane 3), and A-431 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Kinesin 5B Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with KIF5B siRNA and a steep decrease in signal intensity was observed in western blot application using Anti-KIF5B Polyclonal Antibody (Product # PA5-18063). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1-2 µg/mL

Western Blot (WB)

0.5-1.5 µg/mL

Product Specifications

Species Reactivity

Dog, Human

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (QPVAVRGGGGKQV) corresponding to the C-terminus amino acids of KIF5B

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_10984828

Product Specific Information

This antibody is predicted to react with bovine, mouse and rat based on sequence homology.

Target Information

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Conventional kinesin heavy chain; epididymis secretory protein Li 61; kinesin 1 (110-120kD); kinesin family member 5B; kinesin heavy chain; Kinesin-1 heavy chain; U-KHC; Ubiquitous kinesin heavy chain; UKHC

Gene Aliases: HEL-S-61; KIF5B; KINH; KNS; KNS1; UKHC

UniProt ID: (Human) P33176

Entrez Gene ID: (Human) 3799, (Dog) 100855651

Molecular Function: cytoskeletal protein microtubule binding motor protein microtubule family cytoskeletal protein