OriGene

L1CAM Monoclonal Antibody (OTI1H3), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. View Details

View all (68) CD171 (L1CAM) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

L1CAM Antibody in Western Blot (WB) — Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and L1CAM-Knockout HeLa cells (KO, Cat# LC832196) were separated by SDS-PAGE and immunoblotted with anti-L1CAM monoclonal antibody TA500353(1:1000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

L1CAM Antibody in Immunofluorescence (IF) — Anti-L1CAM mouse monoclonal antibody (TA500353) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).

L1CAM Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA500353), and then analyzed by flow cytometry.

L1CAM Antibody in Immunoprecipitation (IP) — Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

L1CAM Antibody in Knockout — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in L1CAM knockout (KO) HeLa cell line using Anti-L1CAM monoclonal antibody (Product # CF500353). {KO}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2500-1:5000

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Flow Cytometry (Flow)

1:100

Immunoprecipitation (IP)

2-4 µg/mg

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1H3

Immunogen

Full length human recombinant protein of human L1CAM produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

UniProt ID: (Human) P32004

Entrez Gene ID: (Human) 3897

Molecular Function: G-protein coupled receptor cell adhesion molecule cytokine receptor defense/immunity protein hydrolase immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule phosphatase protein phosphatase receptor