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Invitrogen
LDHA Polyclonal Antibody
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FIGURE: 1 / 19
LDHA Antibody (PA5-27406) in ICC/IF
Immunofluorescence analysis of LDHA was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with LDHA Polyclonal Antibody (Product # PA5-27406) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a diluti... View More
Product Details
PA5-27406
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human LDHA. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, MCF-7, MDA-MB-231, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.
Predicted reactivity: Mouse (92%), Rat (91%), Xenopus laevis (80%), Pig (90%), Rabbit (91%), Chicken (80%), Rhesus Monkey (99%), Chimpanzee (100%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers. Mutations in the LDHA gene can lead to Glycogen storage disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; L-lactate dehydrogenase; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; Lactate Dehydrogenase; lactate dehydrogenase 1, A chain; lactate dehydrogenase 3, C chain, sperm specific; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase C variant 3; lactate dehydrogenase M; lactate dehydrogenase-A; Lactic Dehydrogenase; LDH; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; M-LDH; OTTHUMP00000165206; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; Ldh3; LDHA; LDHM; PIG19
UniProt ID: (Human) P00338, (Rat) P04642, (Mouse) P06151
Entrez Gene ID: (Human) 3939, (Rat) 24533, (Mouse) 16828, (Rat) 29634
L-lactate dehydrogenase activity
protein binding
kinase binding
identical protein binding
NAD binding
cadherin binding involved in cell-cell adhesion
lactate dehydrogenase activity
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
dehydrogenase
response to hypoxia
lactate metabolic process
pyruvate metabolic process
glycolytic process
response to nutrient
response to glucose
NAD metabolic process
response to drug
response to hydrogen peroxide
positive regulation of apoptotic process
response to estrogen
post-embryonic organ development
response to cAMP
oxidation-reduction process
cell-cell adhesion
response to organic cyclic compound
substantia nigra development
cellular response to extracellular stimulus
cellular carbohydrate metabolic process
carbohydrate metabolic process
lactate biosynthetic process from pyruvate
carboxylic acid metabolic process
ATP biosynthetic process
lactate oxidation
sperm motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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