OriGene

LXN Monoclonal Antibody (OTI2G4), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (23) Latexin antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

LXN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503644)

LXN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human prostate tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503644)

LXN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human thyroid tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503644)

LXN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human colon tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503644)

LXN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503644)

LXN Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (RC202769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN. Positive lysates LY412596 (100 µg) and LC412596 (20 µg) can be purchased separately from OriGene.

LXN Antibody in Western Blot (WB) — Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and LXN-Knockout HeLa cells (KO, Cat# LC812573) were separated by SDS-PAGE and immunoblotted with anti-LXN monoclonal antibody TA503644(1:2000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

LXN Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from a mouse cell line and 2 different mouse tissues by using anti-LXN monoclonal antibody. (1:200)

LXN Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-LXN monoclonal antibody.

LXN Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in LXN knockout (KO) HeLa cell line using Anti-LXN monoclonal antibody (Product # CF503644). {KO}

Product Details

CF503644

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI2G4
Immunogen	Full length human recombinant protein of human LXN produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

This gene encodes the only known protein inhibitor of zinc-dependent metallocarboxypeptidases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ECI; Endogenous carboxypeptidase inhibitor; Latexin; latexin protein; MUM; Protein MUM; TCI; Tissue carboxypeptidase inhibitor

Gene Aliases: ECI; LXN; TCI

UniProt ID: (Human) Q9BS40, (Mouse) P70202

Entrez Gene ID: (Human) 56925, (Mouse) 17035

Molecular Function: protease inhibitor

inflammatory response negative regulation of endopeptidase activity detection of temperature stimulus involved in sensory perception of pain negative regulation of peptidase activity

Process(es)

protein binding metalloendopeptidase inhibitor activity heparin binding enzyme inhibitor activity peptidase inhibitor activity