|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Clone||M2-7C10 + M2-9E3|
|Immunogen||Recombinant hMART-1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14017 targets MART-1 in IHC (P), IP, and WB applications and shows reactivity with Equine and Human samples.
The MA5-14017 immunogen is recombinant hMART-1 protein.
MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-Ais a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ambiguous melanocytic tumors in a tertiary referral center: the contribution of fluorescence in situ hybridization (FISH) to conventional histopathologic and immunophenotypic analyses.
MA5-14017 was used in immunohistochemistry to study the use of FISH as a diagnostic aid in patients with ambiguous melanocytic tumors
|Tetzlaff MT,Wang WL,Milless TL,Curry JL,Torres-Cabala CA,McLemore MS,Ivan D,Bassett RL,Prieto VG||The American journal of surgical pathology (37:1783)||2013|
Expression of the embryological morphogen Nodal in stage III/IV melanoma.
MA5-14017 was used in immunohistochemistry to investigate the role of Nodal during melanoma progression
|Hooijkaas AI,Gadiot J,van Boven H,Blank C||Melanoma research (21:491)||2011|
Cellular blue nevus of the uterus: a case report and review of the literature.
MA5-14017 was used in immunohistochemistry to report on a case of cellular blue nevus of the uterus
|Eskue K,Prieto VG,Malpica A||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (29:583)||2010|
Regulation of human skin pigmentation in situ by repetitive UV exposure: molecular characterization of responses to UVA and/or UVB.
MA5-14017 was used in immunohistochemistry to study the molecular responses of the skin pigmentation system to repeated exposure to UVA and UVB
|Choi W,Miyamura Y,Wolber R,Smuda C,Reinhold W,Liu H,Kolbe L,Hearing VJ||The Journal of investigative dermatology (130:1685)||2010|
Vaccine-specific local T cell reactivity in immunotherapy-associated vitiligo in melanoma patients.
MA5-14017 was used in immunohistochemistry to investigate the association between vitiligo and immunetherapy for melanoma
|Jacobs JF,Aarntzen EH,Sibelt LA,Blokx WA,Boullart AC,Gerritsen MJ,Hoogerbrugge PM,Figdor CG,Adema GJ,Punt CJ,de Vries IJ||Cancer immunology, immunotherapy : CII (58:145)||2009|
Diagnostic challenges of metastatic spindle cell melanoma on fine-needle aspiration specimens.
MA5-14017 was used in immunohistochemistry to review the diagnostic difficulty of metastatic spindle cell melanoma
|Piao Y,Guo M,Gong Y||Cancer (114:94)||2008|
Glomus tumor and glomangioma of the nerve. Report of two cases.
MA5-14017 was used in immunohistochemistry to report on two cases of glomus tumor and glomangioma of the nerve
|Scheithauer BW,Rodriguez FJ,Spinner RJ,Dyck PJ,Salem A,Edelman FL,Amrami KK,Fu YS||Journal of neurosurgery (108:348)||2008|
McCune-Albright syndrome in a boy may present with a monolateral macroorchidism as an early and isolated clinical manifestation.
MA5-14017 was used in immunohistochemistry to report on a case of McCune-Albright syndrome with monolateral macroorchidism
|Arrigo T,Pirazzoli P,De Sanctis L,Leone O,Wasniewska M,Messina MF,De Luca F||Hormone research (65:114)||2006|
Immunogenicity, including vitiligo, and feasibility of vaccination with autologous GM-CSF-transduced tumor cells in metastatic melanoma patients.
MA5-14017 was used in immunohistochemistry to study the therapeutic potential of autologous tumor cells virally transduced with GM-CSF in metastatic melanoma patients
|Luiten RM,Kueter EW,Mooi W,Gallee MP,Rankin EM,Gerritsen WR,Clift SM,Nooijen WJ,Weder P,van de Kasteele WF,Sein J,van den Berk PC,Nieweg OE,Berns AM,Spits H,de Gast GC||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (23:8978)||2005|
Mechanisms of skin tanning in different racial/ethnic groups in response to ultraviolet radiation.
MA5-14017 was used in immunohistochemistry to study the mechanisms by which UV radiation stimulates skin pigmentation in different ethnic groups
|Tadokoro T,Yamaguchi Y,Batzer J,Coelho SG,Zmudzka BZ,Miller SA,Wolber R,Beer JZ,Hearing VJ||The Journal of investigative dermatology (124:1326)||2005|
On the role of melanoma-specific CD8+ T-cell immunity in disease progression of advanced-stage melanoma patients.
MA5-14017 was used in immunohistochemistry to study the role of CD8+ T-cell immunity in disease progression of advanced-stage melanoma
|van Oijen M,Bins A,Elias S,Sein J,Weder P,de Gast G,Mallo H,Gallee M,Van Tinteren H,Schumacher T,Haanen J||Clinical cancer research : an official journal of the American Association for Cancer Research (10:4754)||2004|
Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells.
MA5-14017 was used in western blot to develop human artificial adjuvant vector cells for cancer immunotherapy
|Shimizu K,Mizuno T,Shinga J,Asakura M,Kakimi K,Ishii Y,Masuda K,Maeda T,Sugahara H,Sato Y,Matsushita H,Nishida K,Hanada K,Dorrie J,Schaft N,Bickham K,Koike H,Ando T,Nagai R,Fujii S||Cancer research (73:62)||2013|
Characterization of the bioactive motif of neuregulin-1, a fibroblast-derived paracrine factor that regulates the constitutive color and the function of melanocytes in human skin.
MA5-14017 was used in western blot to study a peptide sequence motif within neuregulin-1 that promotes the biosynthesis of melanin but not melanocyte proliferation
|Choi W,Kolbe L,Hearing VJ||Pigment cell and melanoma research (25:477)||2012|
Glycoprotein nonmetastatic melanoma protein b, a melanocytic cell marker, is a melanosome-specific and proteolytically released protein.
MA5-14017 was used in western blot to study the proteolytic release of glycoprotein nonmetastatic melanoma protein b from melanosomes
|Hoashi T,Sato S,Yamaguchi Y,Passeron T,Tamaki K,Hearing VJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (24:1616)||2010|
|Non-human primate||Not Cited||
Dendritic cell vaccine with mRNA targeted to the proteasome by polyubiquitination.
MA5-14017 was used in western blot to study the therapeutic potential of a dendritic cell vaccine using mRNAs encoding tumor-associated antigens targeted to the proteasome
|Hosoi A,Takeda Y,Sakuta K,Ueha S,Kurachi M,Kimura K,Maekawa R,Kakimi K||Biochemical and biophysical research communications (371:242)||2008|
Cyclobutane pyrimidine dimer formation and p53 production in human skin after repeated UV irradiation.
MA5-14017 was used in immunocytochemistry to study the potential protective effects of facultative pigmentation following repeated exposure to UV irradiation
|Yamaguchi Y,Coelho SG,Zmudzka BZ,Takahashi K,Beer JZ,Hearing VJ,Miller SA||Experimental dermatology (17:916)||2008|
MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes.
MA5-14017 was used in immunoprecipitation to study the role of MART-1 on PMEL17/GP100 function and melanosome maturation
|Hoashi T,Watabe H,Muller J,Yamaguchi Y,Vieira WD,Hearing VJ||The Journal of biological chemistry (280:14006)||2005|
Magnetic bead RT-PCR: establishment of a new method for detecting circulating melanoma cells.
MA5-14017 was used in flow cytometry and immunoprecipitation to develop a novel magnetic-activated cell separation/RT-PCR method for detecting circulating melanoma cell anaylsis
|Georgieva J,Milling A,Orfanos CE,Geilen CC||Melanoma research (12:309)||2002|