Invitrogen

MCM7 Monoclonal Antibody (47DC141 (DCS-141))

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

10 References

View all (32) MCM7 antibodies

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Datasheet

Protocols

Questions & Answers

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MCM7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of MCM7 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLK1 Monoclonal Antibody (36-298) (Product # MA5-14291) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

MCM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded rat breast carcinoma stained with CDC47 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

MCM7 Antibody in Western Blot (WB) — Knockdown of MCM7 was achieved by transfecting Caco-2 with MCM7 specific siRNAs (Silencer® select Product # s8602, s8603). Western blot analysis (Fig. a) was performed using whole cell extracts from the MCM7 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with MCM7 Monoclonal Antibody (47DC141 or DCS-141) (Product # MA5-14291, 2µg/ml) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MCM7.

MCM7 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of CDC47 using CDC47 Monoclonal Antibody (Product # MA5-14291) on Native Human LS174T Cells.

MCM7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with MCM7 siRNA and reduction of signal was observed in Western Blot using MCM7 Monoclonal Antibody (47DC141 or DCS-141) (Product # MA5-14291). {KD}

Product Details

MA5-14291

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1.0 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-
Immunoprecipitation (IP)	2 µg/mg protein lysate	-

Product Specifications
Species Reactivity	Dog, Human, Mouse, Rat, Xenopus
Published species	Dog, Human, Mouse, Rabbit
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	47DC141 (DCS-141)
Immunogen	Recombinant hCDC47 protein
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_11009501

Product Specific Information

MA5-14291 targets CDC47 in IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, mouse, Rat, and Xenopus laevis samples.

The MA5-14291 immunogen is recombinant hCDC47 protein.

Target Information

MCM7 encodes a protein that is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA mediated chromatin pull-down for the study of chromatin replication.

Scientific reports

Kliszczak AE,Rainey MD,Harhen B,Boisvert FM,Santocanale C

MA5-14291 was used in Western Blotting to develop an experimental approach allowing chromatin to be captured, and its content analysed.

Thu Aug 01 00:00:00 EDT 2013

Synonymous codon changes in the oncogenes of the cottontail rabbit papillomavirus lead to increased oncogenicity and immunogenicity of the virus.

Virology

Cladel NM,Budgeon LR,Hu J,Balogh KK,Christensen ND

MA5-14291 was used in immunohistochemistry to study the increased oncogenicity and immunogenicity of the cottontail rabbit papillomavirus following synonymous mammalian-like changes in codon usage

Wed Apr 10 00:00:00 EDT 2013

Encapsulation of cisplatin in long-circulating and pH-sensitive liposomes improves its antitumor effect and reduces acute toxicity.

International journal of nanomedicine

Leite EA,Souza CM,Carvalho-Júnior AD,Coelho LG,Lana AM,Cassali GD,Oliveira MC

MA5-14291 was used in immunohistochemistry to study the delivery of cisplatin via pH-sensitive liposomes with a long circulating lifespan to increase antitumor activity whilst reducing acute toxicity

Mon Apr 08 00:00:00 EDT 2013

Evaluation of minichromosome maintenance protein 7 as a prognostic marker in canine cutaneous mast cell tumours.

Veterinary and comparative oncology

Berlato D,Stewart J,Newton R,Maglennon GA,Monti P,Flindall A,Murphy S

MA5-14291 was used in immunohistochemistry to study the prognostic value of the immunohistochemical expression of MCM7 in canine cutaneous mast cell tumours

Fri Jun 01 00:00:00 EDT 2012

Persistence of high-grade cervical dysplasia and cervical cancer requires the continuous expression of the human papillomavirus type 16 E7 oncogene.

Cancer research

Jabbar SF,Abrams L,Glick A,Lambert PF

MA5-14291 was used in immunohistochemistry to study the requirement for continuous expression of the E7 protein of HPV type 16 for the maintenance of high-grade cervical dysplasia and cervical cancer

Fri May 15 00:00:00 EDT 2009

Cell proliferation index predicts relapse of brain metastases in non-irradiated patients.

Acta neurochirurgica

Peev NA,Tonchev AB,Penkowa M,Kalevski SK,Haritonov DG,Chaldakov GN

MA5-14291 was used in immunohistochemistry to study the value of proliferative index in predicting brain metastases relapse in non-irradiated patients

Wed Oct 01 00:00:00 EDT 2008

Critical roles for non-pRb targets of human papillomavirus type 16 E7 in cervical carcinogenesis.

Cancer research

Balsitis S,Dick F,Dyson N,Lambert PF

MA5-14291 was used in immunohistochemistry to investigate the effect of non-pRb targets of papillomavirus type 16 E7 on cervical carcinogenesis

Sun Oct 01 00:00:00 EDT 2006

Oxidative stress regulated expression of ubiquitin Carboxyl-terminal Hydrolase-L1: role in cell survival.

Apoptosis : an international journal on programmed cell death

Shen H,Sikorska M,Leblanc J,Walker PR,Liu QY

MA5-14291 was used in western blot to study the role of oxidative stress on ubiquitin carboxy-terminal hydrolase L1 expression and embryonal carcinoma cell survival

Thu Jun 01 00:00:00 EDT 2006

Repression of DNA replication licensing in quiescence is independent of geminin and may define the cell cycle state of progenitor cells.

Experimental cell research

Kingsbury SR,Loddo M,Fanshawe T,Obermann EC,Prevost AT,Stoeber K,Williams GH

MA5-14291 was used in western blot to study the role of geminin in the DNA replication licensing during quiescence of progenitor cells

Sat Sep 10 00:00:00 EDT 2005

Human cytomegalovirus prevents replication licensing by inhibiting MCM loading onto chromatin.

EMBO reports

Wiebusch L,Uecker R,Hagemeier C

MA5-14291 was used in western blot to study the mechanism by which human cytomegalovirus prevents replication licensing

Wed Jan 01 00:00:00 EST 2003

Bioinformatics

Protein Aliases: CDC47 homolog; CDC47 homolog A; CDC47p; DNA replication licensing factor MCM7; DNA replication licensing factor mcm7-A; homolog of S. cerevisiae Cdc47; mini chromosome maintenance deficient 7; minichromosome maintenance deficient 7; minichromosome maintenance protein 7; Minichromosome maintenance protein 7-A; P1 P85MCM; P1.1-MCM3; p90; protein phosphatase 1, regulatory subunit 104; XMCM7; xMCM7-A

Gene Aliases: AI747533; CDC47; mCDC47; MCM2; MCM7; mcm7-a; mcm7-b; Mcmd7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104; XELAEV_18000877mg

UniProt ID: (Human) P33993, (Mouse) Q61881, (Xenopus) Q91876

Entrez Gene ID: (Dog) 479737, (Human) 4176, (Mouse) 17220, (Rat) 288532, (Xenopus) 397852

Molecular Function: DNA metabolism protein

Function(s)

G1/S transition of mitotic cell cycle DNA replication DNA unwinding involved in DNA replication DNA replication initiation cellular response to DNA damage stimulus cell proliferation regulation of phosphorylation response to drug cellular response to epidermal growth factor stimulus cellular response to xenobiotic stimulus cell cycle regulation of transcription, DNA-templated cellular response to organic substance

Process(es)

DNA binding single-stranded DNA binding ATP-dependent DNA helicase activity protein binding ATP binding nucleotide binding DNA helicase activity helicase activity hydrolase activity DNA-dependent ATPase activity

MCM7 Monoclonal Antibody (47DC141 (DCS-141))

Product Details

MA5-14291

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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