Immunofluorescence analysis of MEK1 was done on 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with MEK1 Mouse Monoclonal Antibody (133500) at 2 µg - 4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 Rabbit Anti-Mouse IgG Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization of MEK1. Panel e shows no primary antibody. The images were captured at 20X magnification.
|Tested species reactivity||Dog, Human, Mouse, Non-human primate, Rat, Xenopus|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified MEK from human T cells and recombinant MEK1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MAP2K1 is a dual specificity mitogen-activated protein kinase (MAPK) kinase. It is an essential component of the MAP kinase pathway and stimulates the enzymatic activity of MAP kinases in response to various signals. Growth factors, cytokines, and proto-oncogenes transduce their signals through the activation of RAS, which leads to activation of the serine/threonine kinase RAF, and then to activation of MAPK kinase. MAP2K1 is activated by the Raf family member p74raf-1, through phosphorylation of serine residues at positions 217 and 221 in the activation loop.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PTPIP51¿A New RelA-tionship with the NF¿B Signaling Pathway.
13-3500 was used in proximity ligation assay to elucidate how protein tyrosine phosphatase interacting protein 51 modulates the NF-kappaB signaling pathway.
|Brobeil A,Kämmerer F,Tag C,Steger K,Gattenlöhner S,Wimmer M||Biomolecules (5:485)||2015|
|Mouse||Not Cited||Expression of Galpha 13 (Q226L) induces P19 stem cells to primitive endoderm via MEKK1, 2, or 4.||Wang HY,Kanungo J,Malbon CC||The Journal of biological chemistry (277:3530)||2002|
|Mouse||Not Cited||An analysis of Mek1 signaling in cell proliferation and transformation.||Greulich H,Erikson RL||The Journal of biological chemistry (273:13280)||1998|