Immunohistochemistry analysis of MMP-9 showing staining in the cytoplasm of paraffin-embedded human colon carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a MMP-9 monoclonal antibody (Product # MA5-14228) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Dog, Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human native 92kDa MMP-9|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14228 targets MMP-9 (92kDa Collagenase IV) in IHC, IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-14228 immunogen is human native 92kDa MMP-9.
MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and inhibition by specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). Reportedly, MMPs play a crucial role in tumor cell invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The pattern of CD44 and matrix metalloproteinase 9 expression is a useful predictor of ulcerative colitis-associated dysplasia and neoplasia.
MA5-14228 was used in immunohistochemistry - paraffin section to examine CD44 and MMP-9 expression in different ulcerative colitis lesions
|AbdElazeem MA,El-Sayed M||Annals of diagnostic pathology (19:369)||2015|
Recruitment of Matrix Metalloproteinase-9 (MMP-9) to the Fibroblast Cell Surface by Lysyl Hydroxylase 3 (LH3) Triggers Transforming Growth Factor-ß (TGF-ß) Activation and Fibroblast Differentiation.
MA5-14228 was used in immunocytochemistry and western blot to study the recruitment of MMP-9 to cell surface and its effect on fibroblast differentiation
|Dayer C,Stamenkovic I||The Journal of biological chemistry (290:13763)||2015|
Biological and clinical relevance of matrix metalloproteinases 2 and 9 in acute myeloid leukaemias and myelodysplastic syndromes.
MA5-14228 was used in immunocytochemistry to study the biological and clinical relevance of MMP2 and MMP9 in acute myeloid leukaemias and myelodysplastic syndromes
|Travaglino E,Benatti C,Malcovati L,Della Porta MG,Gallì A,Bonetti E,Rosti V,Cazzola M,Invernizzi R||European journal of haematology (80:216)||2008|
In vivo effects of zoledronic acid on oral mucosal epithelial cells.
MA5-14228 was used in immunohistochemistry to investigate the influence of zoledronic acid on oral mucosal epithelial cells in a dog model
|Allam E,Allen M,Chu TM,Ghoneima A,Jack Windsor L||Oral diseases (17:291)||2011|
Noninvasive limb ischemic preconditioning protects against myocardial I/R injury in rats.
MA5-14228 was used in immunohistochemistry to compare the protective effect of noninvasive limb ischemia preconditioning against myocardial ischemia/reperfusion injury in rats
|Li SJ,Wu YN,Kang Y,Yin YQ,Gao WZ,Liu YX,Lou JS||The Journal of surgical research (164:162)||2010|
Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming.
MA5-14228 was used in immunohistochemistry to investigate the mechanism for the non-responsiveness to prostaglandin priming in cervix of postterm women
|Sahlin L,Stjernholm-Vladic Y,Roos N,Masironi B,Ekman-Ordeberg G||Reproductive biology and endocrinology : RB and E (6:null)||2008|
Pathogenic sequence for dissecting aneurysm formation in a hypomorphic polycystic kidney disease 1 mouse model.
MA5-14228 was used in immunohistochemistry to study vascular remodeling in a hypomorphic Pkd1(nl/nL) mouse model with dissecting aneurysms in the aorta
|Hassane S,Claij N,Lantinga-van Leeuwen IS,Van Munsteren JC,Van Lent N,Hanemaaijer R,Breuning MH,Peters DJ,DeRuiter MC||Arteriosclerosis, thrombosis, and vascular biology (27:2177)||2007|
Time course of matrix metalloproteases and tissue inhibitors in bleomycin-induced pulmonary fibrosis.
MA5-14228 was used in immunohistochemistry to investigate the distribution, expression and activity of matrix metalloproteases and tissue inhibitors during extracellular matrix remodeling in pulmonary fibrosis
|Oggionni T,Morbini P,Inghilleri S,Palladini G,Tozzi R,Vitulo P,Fenoglio C,Perlini S,Pozzi E||European journal of histochemistry : EJH (50:317)||2007|
Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.
MA5-14228 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae
|Kirkegaard T,Hansen A,Bruun E,Brynskov J||Gut (53:701)||2004|
Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts.
MA5-14228 was used in western blot to study the effects of fibroblasts on mammary carcinoma cells.
|Olsen CJ,Moreira J,Lukanidin EM,Ambartsumian NS||BMC cancer (10:null)||2010|
Cardosins improve neuronal regeneration after cell disruption: a comparative expression study.
MA5-14228 was used in western blot to study the use of the plant enzyme cardosin for tissue disaggregation and isolation of rat embryonic neurons
|Duarte AS,Duarte EP,Correia A,Pires E,Barros MT||Cell biology and toxicology (25:99)||2009|
Improved surgical mesh integration into the rat abdominal wall with arginine administration.
MA5-14228 was used in western blot to study the role of arginine administration in improving surgical mesh integration into the rat abdominal wall
|Arbos MA,Ferrando JM,Quiles MT,Vidal J,López-Cano M,Gil J,Manero JM,Peña J,Huguet P,Schwartz-Riera S,Reventós J,Armengol M||Biomaterials (27:758)||2006|
Simvastatin suppresses experimental aortic aneurysm expansion.
MA5-14228 was used in western blot to study the therapeutic benefits of simvastatin against experimental aortic aneurysm expansion
|Kalyanasundaram A,Elmore JR,Manazer JR,Golden A,Franklin DP,Galt SW,Zakhary EM,Carey DJ||Journal of vascular surgery (43:117)||2006|
Effect of gamma-synuclein overexpression on matrix metalloproteinases in retinoblastoma Y79 cells.
MA5-14228 was used in western blot to study the effect of gamma-synuclein overexpression on MMPs in retinoblastoma cells
|Surgucheva IG,Sivak JM,Fini ME,Palazzo RE,Surguchov AP||Archives of biochemistry and biophysics (410:167)||2003|