OriGene

MYD88 Monoclonal Antibody (OTI1B8), TrueMAB™

View all (31) MyD88 antibodies

Questions & Answers

Questions & Answers

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MYD88 Antibody in Immunocytochemistry (ICC/IF) — Anti-MYD88 mouse monoclonal antibody (TA502118) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88(RC202253). (1:100)

MYD88 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88(RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88. (1:500)

MYD88 Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 3 cell lines lysates by using anti-MYD88 monoclonal antibody. (1:500)

MYD88 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC202253 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody (TA502118), and then analyzed by flow cytometry. (1:100)

Product Details

TA502118

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI1B8
Immunogen	Full length human recombinant protein of human MYD88 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.4 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL-1R and is involved in the inflammatory response induced by IL-1, IL-18 and LPS. MyD88 recruits IRAK to IL-1 receptor complex in response to IL-1. This pathway further leads to activation of NF-kB. MyD88 has a wide tissue distribution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: mutant myeloid differentiation primary response 88; myeloid differentiation primary response gene (88); myeloid differentiation primary response gene 88; Myeloid differentiation primary response protein MyD88

Gene Aliases: MYD88; MYD88D

UniProt ID: (Human) Q99836, (Rat) Q6Y1S1

Entrez Gene ID: (Human) 4615, (Rat) 301059

Molecular Function: scaffold/adaptor protein

toll-like receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway apoptotic process inflammatory response signal transduction cell surface receptor signaling pathway positive regulation of type I interferon production positive regulation of interleukin-17 production positive regulation of interleukin-23 production positive regulation of interleukin-6 production toll-like receptor 9 signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity response to interleukin-1 3'-UTR-mediated mRNA stabilization cellular response to mechanical stimulus response to molecule of fungal origin JNK cascade Toll signaling pathway response to virus immunoglobulin mediated immune response cytokine-mediated signaling pathway lipopolysaccharide-mediated signaling pathway response to peptidoglycan response to lipopolysaccharide positive regulation of tumor necrosis factor production regulation of cell proliferation negative regulation of growth of symbiont in host positive regulation of chemokine biosynthetic process type I interferon biosynthetic process positive regulation of JNK cascade positive regulation of smooth muscle cell proliferation positive regulation of lymphocyte proliferation establishment of endothelial intestinal barrier

Process(es)

death receptor binding protein binding identical protein binding protein self-association TIR domain binding receptor binding Toll binding interleukin-1 receptor binding