Invitrogen

MYH10 Monoclonal Antibody (GT246)

MYH10 Antibody in Immunocytochemistry (ICC/IF)

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MYH10 Antibody (MA5-27767) in ICC/IF

MYH10 Monoclonal Antibody (GT246) detects MYH10 protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MYH10 protein stained by MYH10 Monoclonal Antibody (GT246) (Product # MA5-27767) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

MYH10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MYH10 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA5-27767

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

GT246

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MYH10. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735278

Product Specific Information

Positive Control: HeLa, MYH10-transfected 293T, NIH-3T3, mouse brain

Predicted Reactivity: Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

MYH10 encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Atp7b-dependent choroid plexus dysfunction causes transient copper deficit and metabolic changes in the developing mouse brain.

PLoS genetics

Washington-Hughes CL,Roy S,Seneviratne HK,Karuppagounder SS,Morel Y,Jones JW,Zak A,Xiao T,Boronina TN,Cole RN,Bumpus NN,Chang CJ,Dawson TM,Lutsenko S

MA5-27767 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to identify the new role for Atp7b in developing brain and identify metabolic changes that could be exacerbated by Cu chelation therapy.

Sun Jan 01 00:00:00 EST 2023

Bioinformatics

Protein Aliases: Cellular myosin heavy chain, type B; flectin; MCH-B(B2); Myosin heavy chain 10; myosin heavy chain 10, non-muscle; Myosin heavy chain, non-muscle IIb; myosin heavy chain, nonmuscle type B; myosin heavy chain, nonmuscular, type B; myosin IIB; myosin, heavy polypeptide 10, non-muscle; Myosin-10; NMMHC II-b; NMMHC IIB; NMMHC-B; NMMHC-IIB; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; nonmuscle myosin; nonmuscle myosin heavy chain II-B; nonmuscle myosin heavy chain IIB; nonmuscle myosin heavy chain-B; nonmuscle myosin II heavy chain-B; OTTHUMP00000165799

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Gene Aliases: 5730504C04Rik; 9330167F11Rik; Fltn; MCH-B; mKIAA3005; MYH10; Myhn-2; Myhn2; NMHC II-B; NMHC-B; NMHCII-B; NMMHC II-b; NMMHC-B; NMMHC-IIB; NMMHCB; SMemb

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UniProt ID: (Human) P35580, (Mouse) Q61879, (Rat) Q9JLT0

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Entrez Gene ID: (Human) 4628, (Mouse) 77579, (Rat) 79433

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Function(s)

microfilament motor activity actin binding protein binding calmodulin binding ATP binding actin-dependent ATPase activity ADP binding actin filament binding nucleotide binding motor activity

Process(es)

mitotic cytokinesis in utero embryonic development neuron migration plasma membrane repair exocytosis substrate-dependent cell migration, cell extension nuclear migration cell adhesion axonogenesis axon guidance brain development adult heart development metabolic process cell proliferation regulation of cell shape fourth ventricle development lateral ventricle development third ventricle development cerebellar Purkinje cell layer development actin cytoskeleton organization actin filament-based movement myofibril assembly actomyosin structure organization neuron projection development neuromuscular process controlling balance cardiac myofibril assembly ventricular cardiac muscle cell development retina development in camera-type eye cardiac septum development aorta development coronary vasculature development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MYH10 Monoclonal Antibody (GT246)

MYH10 Antibody (MA5-27767) in ICC/IF

Product Details

MA5-27767

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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