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Invitrogen
Moesin Polyclonal Antibody
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FIGURE: 1 / 16
Moesin Antibody (PA5-34666) in ICC/IF
Immunofluorescence analysis of Moesin was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Moesin Polyclonal Antibody (Product # PA5-34666) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-34666
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.21 mg/mL
Amount
21 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: NT2D1, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12.
Predicted reactivity: Mouse (98%), Rat (97%), Pig (98%), Rhesus Monkey (100%), Bovine (99%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Moesin, a member of the talin-4. 1 superfamily, is a linking protein of the submembraneous actin cytoskeleton. It is expressed in macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. It is involved in cell adhesion, migration, and organization of cell surface structures.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis luminal protein 70; membrane-organizing extension spike protein; moesin homolog; unnamed protein product
Gene Aliases: HEL70; IMD50
UniProt ID: (Human) P26038, (Mouse) P26041, (Rat) O35763
Entrez Gene ID: (Human) 4478, (Mouse) 17698, (Rat) 81521
immunological synapse formation
leukocyte cell-cell adhesion
regulation of cell shape
regulation of cell size
positive regulation of gene expression
gland morphogenesis
membrane to membrane docking
T cell proliferation
establishment of epithelial cell apical/basal polarity
positive regulation of protein catabolic process
leukocyte migration
establishment of endothelial barrier
T cell aggregation
positive regulation of podosome assembly
T cell migration
regulation of organelle assembly
positive regulation of protein localization to early endosome
regulation of lymphocyte migration
positive regulation of early endosome to late endosome transport
response to alcohol
cytoskeleton organization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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