|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Recombinant mouse GM-CSF|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MM500C targets Mouse GM-CSF in ELISA, and WB applications and shows reactivity with mouse samples.
The MM500C immunogen is recombinant mouse GM-CSF.
MM500C detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa.
The MM500C mouse GMCSF antibody (clone 22E9) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM500DB (biotinylated conjugate of clone 31G6) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody MM500C (clone 22E9) and biotinylated antibody MM500DB (clone 31G6) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The water-soluble extract from cultured medium of Ganoderma lucidum (Reishi) mycelia (Designated as MAK) ameliorates murine colitis induced by trinitrobenzene sulphonic acid.
MM500C was used in blocking or activating experiment to study the beneficial effect of a water-soluble, polysaccharide components of Reishi in a mouse model of colitis and the potential mechanism
|Hanaoka R,Ueno Y,Tanaka S,Nagai K,Onitake T,Yoshioka K,Chayama K||Scandinavian journal of immunology (74:454)||2011|
Humoral and cellular immunity in secondary infection due to murine Chlamydia trachomatis.
MM500C was used in blocking/activating experiment to study the role of murine Chlamydia trachomatis in secondary infection
|Williams DM,Grubbs BG,Pack E,Kelly K,Rank RG||Infection and immunity (65:2876)||1997|
Combination of Fasl and GM-CSF confers synergistic antitumor immunity in an in vivo model of the murine Lewis lung carcinoma.
MM500C was used in western blot to study whether combined FasL and GM-CSF treatment can efficiently suppress tumor growth in Lewis lung carcinoma
|Ho MY,Sun GH,Leu SJ,Ka SM,Tang SJ,Sun KH||International journal of cancer (123:123)||2008|
Potentiation by granulocyte macrophage colony-stimulating factor of lipopolysaccharide toxicity in mice.
MM500C was used in western blot to study the LPS toxicity potentiation by GM-CSF in mice
|Tiegs G,Barsig J,Matiba B,Uhlig S,Wendel A||The Journal of clinical investigation (93:2616)||1994|