This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details
MA3-941 detects mu-calpain from human platelets and erythrocytes, bovine platelets, heart and skeletal muscle, rat myoblasts, kidney, liver and spleen, and pig cultured cells. This antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin.
MA3-941 has been successfully used in Western blot, IF and immunocytochemistry procedures. By Western blot, this antibody detects an ~80 kDa protein representing mu-calpain from human platelets and erythrocytes. Immunocytochemical staining of mu-calpain in porcine LLC-PK1 cells with MA3-941 results in diffuse cytoplasmic staining. This product has not been shown to be effective in immunoprecipitation experiments.
The MA3-941 antigen is purified bovine skeletal muscle 80 kDa mu-calpain subunit. This antibody recognizes an epitope between amino acids 245-265 (domain II) of human mu-calpain.
The calpains including Calpain 1 (CAPN1), calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. Diseases associated with CAPN1 include Spastic Paraplegia 76, Autosomal Recessive and Spasticity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain-I; Calpain-II; CANP 1; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; Micromolar-calpain; mu-calpain; muCANP
Gene Aliases: BOS_24946; CANP; CANP1; CANPL1; CAPN1; Cls1; muCANP; muCL; PIG30; SPG76