Invitrogen

Myogenin Monoclonal Antibody (5FD)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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Myogenin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human myocytes using a Myogenin monoclonal antibody (Product # MA1-41042) at 2.5 µg/mL. Hematoxylin-eosin counterstain.

Myogenin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of a frozen tissue section ofhuman alveolar rhabdomyosarcoma using a Myogenin monoclonal antibody (Product # MA1-41042) at5 µg/mL.

Myogenin Antibody in Western Blot (WB) — Western blot was performed using Anti-Myogenin Monoclonal Antibody (5FD) (Product # MA1-41042) and a 30 kDa band corresponding to MYOG was observed across cell lines except HEK-293 and NIH/3T3 which are reported to be negative and also increased upon 2% heat inactivated horse serum treatment. Modified whole cell extracts (1% SDS) (30 µg lysate) of C2C12 (Lane 1), C2C12 treated with 2% heat inactivated horse serum for 5days (Lane 2), RD (Lane 3), L6 (Lane 4), HEK-293 (Lane 5) and NIH/3T3 (Lane 6) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Myogenin Antibody in Western Blot (WB) — Western blot analysis of Myogenin expression in various small-round cell tumor lines using a Myogenin monoclonal antibody (Product # MA1-41042). a Myogenin monoclonal antibody (Product # MA1-41042) strongly reacted with a band of appox. 34 kDa in alveolar rhobdomyosarcoma line (Rh30). All other small-round cell tumor lines were negative. PFSK-1 (a primitive neuroectodermal tumor line), EB2 (a lymphoma line), SK-N-SH (a neuroblastoma line), SJSA-1 (Ewing's sarcoma line).

Myogenin Antibody in Cell Treatment — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Myogenin Monoclonal Antibody (5FD) (Product # MA1-41042), shows increased expression of proteins in C212 upon treatment with 2% heat inactivated horse serum for 5 days. {TM}

Myogenin Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of MYOG was observed in RD and L6 in comparison to HEK-293 and NIH/3T3 using Anti-Myogenin Monoclonal Antibody (5FD) (Product # MA1-41042) in Western Blot. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

5-10 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

Western Blot (WB)

2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5FD

Immunogen

Recombinant GST tagged fusion protein corresponding to residues 30-224 of rat myogenin.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2148888

Product Specific Information

Suggested positive control: Rh-30.

Target Information

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bHLHc3; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOD1-related protein; Myogenic factor 4; MYOGENIN

Gene Aliases: BHLHC3; myf-4; MYF4; myo; MYOG

UniProt ID: (Human) P15173, (Mouse) P12979, (Rat) P20428

Entrez Gene ID: (Human) 4656, (Mouse) 17928, (Rat) 29148

Molecular Function: basic helix-loop-helix transcription factor nucleic acid binding transcription factor

ossification cell cycle skeletal muscle tissue development negative regulation of cell proliferation response to gravity positive regulation of myotube differentiation skeletal muscle atrophy positive regulation of muscle atrophy regulation of skeletal muscle satellite cell proliferation response to muscle activity involved in regulation of muscle adaptation response to electrical stimulus involved in regulation of muscle adaptation striated muscle atrophy response to denervation involved in regulation of muscle adaptation skeletal muscle cell differentiation muscle cell fate commitment mRNA transcription from RNA polymerase II promoter skeletal muscle tissue regeneration myoblast differentiation positive regulation of myoblast differentiation negative regulation of glycolytic process positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation positive regulation of cell cycle arrest cellular response to magnetism cellular response to lithium ion cellular response to retinoic acid cellular response to growth factor stimulus cellular response to estradiol stimulus regulation of myoblast fusion positive regulation of myoblast fusion positive regulation of oxidative phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development muscle organ development myotube differentiation cell differentiation histone H3 acetylation histone H4 acetylation positive regulation of transcription, DNA-templated response to muscle activity

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding chromatin DNA binding sequence-specific DNA binding protein heterodimerization activity E-box binding core promoter proximal region sequence-specific DNA binding DNA binding protein dimerization activity molecular_function