Invitrogen

Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor 488, eBioscience™

Product Image Gallery

Myosin 4 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry of differentiated C2C12 cells using 5 µg/mL of Mouse IgG2b Isotype Control Alexa Fluor® 488 (Product # 53-4732-80) (left) or 5 µg/mL Anti-Myosin Heavy Chain Alexa Fluor® 488 (right). Myosin is positive in a subpopulation of differentiated cells. Nuclei are counterstained with DAPI.

Myosin 4 Antibody — Figure 1 TAK-242 reduces LPS-induced atrophy of C2C12 myotubes. ( A,B ) Western blot analysis ( A ) and quantification ( B ) of MyHC expression in C2C12 myotubes treated for 48 h with vehicle, LPS (1 mug/mL), or LPS and TAK-242 (1 muM). Data in ( B ) were normalised to beta-tubulin protein levels, and the ratio in vehicle-treated control cells was set to 1. N = 5/group. Full-length blots are presented in Supplementary Figure S4. ( C ) Representative immunofluorescence staining of MyHC in C2C12 myotubes treated as described for ( A,B ). Scale bar = 100 mum. ( D-F ) Distribution of myotube widths ( D ), mean myotube width ( E ), and fusion index ( F ) of C2C12 cells treated as described in ( A,B ). Myotube width was measured as described in the Methods. N = 97-114. The fusion index was calculated from five randomly selected fields as described in the Methods. For all panels, data are presented as the mean +- s.e.m. ***p < 0.001, **p < 0.01, *p < 0.05 vs vehicle control; ### p < 0.001, ## p < 0.01 vs LPS-treated group. P-values were derived from one-way ANOVA followed by Tukey's honest significant difference test or Kruskal-Wallis test followed by Dunn's post hoc tests with Bonferroni correction.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

5 µg/mL

View 8 publications 8 publications

Immunofluorescence (IF)

5 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Western Blot (WB)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Chimpanzee, Cat, Guinea pig, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Human, Mouse

Host / Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MF20

Conjugate

Alexa Fluor® 488

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10671272

Product Specific Information

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.

The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.

Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.

Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product

Gene Aliases: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf

UniProt ID: (Human) Q9Y623, (Mouse) Q5SX39, (Rat) Q29RW1

Entrez Gene ID: (Human) 4622, (Bovine) 408020, (Dog) 479502, (Rabbit) 100009548, (Rabbit) 100125991, (Guinea pig) 100726456, (Mouse) 17884, (Rat) 360543

Molecular Function: G-protein modulator actin binding motor protein actin family cytoskeletal protein cell junction protein cytoskeletal protein enzyme modulator