Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.
The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.
Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Muscle contraction. Weiss A., J. Mol. Biol. 290:61-75(1999). Denis N.J., Proteomics 7:868-874(2007).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2B myosin heavy chain; fast myosin heavy chain 2B; MYH2B; myh4; MyHC-2B; MyHC-IIb; Myosin heavy chain 2b; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain isoform 2b; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4
Gene Aliases: AI506973; BOS_18821; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf
UniProt ID: (Human) Q9Y623, (Dog) Q076A5, (Mouse) Q5SX39, (Rat) Q29RW1
Entrez Gene ID: (Rabbit) 100009548, (Human) 4622, (Bovine) 408020, (Dog) 479502, (Guinea pig) 100726456, (Mouse) 17884, (Rat) 360543
Molecular Function:
G-protein modulator
actin binding motor protein
actin family cytoskeletal protein
cell junction protein
cytoskeletal protein
enzyme modulator
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support
