Invitrogen

N-cadherin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

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N-cadherin Antibody in Immunocytochemistry (ICC/IF) — N-cadherin Polyclonal Antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-cadherin Polyclonal Antibody (Product # PA5-85495) diluted at 1:500. Blue: Hoechst 33342 staining.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — N-cadherin Polyclonal Antibody detects N-Cadherin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. N-Cadherin stained by N-cadherin Polyclonal Antibody (Product # PA5-85495) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

N-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — N-cadherin Polyclonal Antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-cadherin Polyclonal Antibody (Product # PA5-85495) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of N-cadherin in mouse tissue extract using N-cadherin polyclonal antibody (Product # PA5-85495) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

N-cadherin Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in N-cadherin knockout (KO) 293T cell line using Anti-N-cadherin Polyclonal Antibody (Product # PA5-85495). {KO}

Product Details

PA5-85495

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunohistochemistry (Frozen) (IHC (F))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.15 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792635

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Chicken (100%), Bovine (100%).

Positive Control: 293T, NT2D1, SK-N-SH, mouse brain, rat brain, A549.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

Molecular Function: cadherin