Invitrogen

N-cadherin Polyclonal Antibody

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N-cadherin Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of N-cadherin in 4% paraformaldehyde-fixed SH-SY5Y cells using N-cadherin polyclonal antibody (Product # PA5-85495) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of N-cadherin in paraffin-embedded human hepatoma tissue using N-cadherin polyclonal antibody (Product # PA5-85495) at a dilution of 1:500.

N-cadherin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of N-cadherin in frozen E13.5 rat brain using N-cadherin polyclonal antibody (Product # PA5-85495) at a dilution of 1:250. Sample was then incubated with beta Tubulin 3/ TUJ1 (red) and DAPI secondary antibody at a dilution of 1:500.

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of N-cadherin in mouse tissue extract using N-cadherin polyclonal antibody (Product # PA5-85495) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.72 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Keep as concentrated solution.

Positive Control: 293T, NT2D1, SK-N-SH, mouse brain, rat brain, A549.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cadherin 2; cadherin-2 antibody; calcium-dependent adhesion protein; CD325 antibody; CDH2 antibody; CDHN antibody; CDw325 antibody; N-cadherin 1 antibody; NCAD antibody; neural cadherin antibody; cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; N-cadherin; N-cadherin (neuronal) antibody; N-cadherin 1; Neural cadherin; neural-cadherin antibody; neuronal antibody; type 1

Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

Molecular Function: cadherin