Invitrogen

N-cadherin Monoclonal Antibody (5D5)

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

4 Published Figures

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N-cadherin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of A431 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (Green). Blue: DRAQ5 fluorescent DNA dye.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer (D), using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) followed with DAB staining.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human placenta tissues using CD325/N Cadherin monoclonal antibody (Product # MA5-15633).

N-cadherin Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extract (30 µg lysate) of Mouse Brain (Lane 1). The blot was probed with Anti-N-cadherin Monoclonal Antibody (Product # MA5-15633, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 130 kDa band corresponding to N-cadherin was observed in the tissue tested.

N-cadherin Antibody in Western Blot (WB) — Western blot analysis of CD325/N Cadherin using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) in A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

N-cadherin Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of PC-2 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (green) and negative control (purple).

N-cadherin Antibody in Western Blot (WB) — Figure 8 CPPs induce endothelial activation and endothelial-to-mesenchymal transition. ( A ) HCAECs and HITAECs were cultured in the presence of PBS, MPPs, CPP-P, or CPP-S (100 muL of particles per well of a 6-well plate, OD 650 = 0.08-0.10, for CPP-P and CPP-S: equal to 50 mug calcium or 1.2 x 10 5 OsteoSense 680EX-positive PKH67-negative events) for 4 h, and total RNA was extracted with the following expression profiling for the indicated genes (n = 3 wells per group). Heat map shows differentially expressed genes (fold change > 2) between groups. ( B ) HCAECs and HITAECs were cultured in the presence of PBS, MPPs, CPP-P, or CPP-S (100 muL of particles per well of a 6-well plate, OD 650 = 0.08-0.10, for CPP-P and CPP-S: equal to 50 mug calcium or 1.2 x 10 5 OsteoSense 680EX-positive PKH67-negative events) for 4 h. Conditioned media were profiled for interleukin-6 and -8 using an enzyme-linked immunosorbent assay (n = 6 wells per group). Each dot represents one well of the culture plate. Whiskers indicate the range, box bounds indicate the 25th-75th percentiles, and centre lines indicate the median. p -values are provided above the boxes (statistically significant differences are marked by asterisks), Kruskal-Wallis test with post hoc false discovery rate correction by the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli. ( C ) Conditioned media and cell lysate from HCAECs and HITAECs from the experiment in ( B ) were profiled using a cytokine array. N

N-cadherin Antibody in Western Blot (WB) — Figure 4 Systemic treatment with CPPs probably provokes EndoMT in rat endothelium. ( A ) Western blotting measurements of EndoMT transcription factors Snail and Slug and EndoMT marker N-cadherin as compared to the expression of vimentin, CD31 and VE-cadherin in HCAEC and HITAEC co-incubated with PBS, MPP, CPP-P or CPP-S in a flow system for 4 h. Blot scans (top) and band densitometry analysis (bottom). The results of the latter are represented by a heat map. Green, gray and red colours mean fold change <= 0.75, 0.76-1.24, and >=1.25, respectively, compared to PBS group; ( B ) gene expression analysis of SNAI1 , SNAI2 , TWIST1 , ZEB1 , CDH2 , ACTA2 , KDR , PECAM1 and CDH5 genes in HCAEC and HITAEC co-incubated with PBS, MPP, CPP-P or CPP-S in a flow system for 4 h. RT-qPCR measurements, the results are represented by a heat map. Green, gray and red colours mean fold change <= 0.50, 0.51-1.99, and >=2.00, respectively, compared to PBS group; ( C ) Western blotting measurements of EndoMT transcription factors Snail, Slug and Twist1 and alpha-smooth muscle actin as compared to the expression of Cd31, Gapdh and histone H3 in the endothelial lysate collected from the descending aorta and aortic arch of Wistar rats which received consecutive tail vein injections of CPP-P, CPP-S or 0.9% NaCl (10 daily injections). Blot scans (left) and band densitometry analysis (right). The results of the latter are represented by a heat map. Green, gray and red colours mean fold change <= 0.75, 0.7

N-cadherin Antibody in Western Blot (WB) — Figure 1 Profiling of key endothelial molecules in human coronary artery endothelial cells (HCAECs) co-cultured with either human internal thoracic artery endothelial cells (HITAECs) (HIT) or human saphenous vein endothelial cells (HSaVECs) (HSVs) for 6, 24, or 48 h. ( A ) RT-qPCR profiling, HIT signifies the ratio of transcript levels (measured as DeltaCt) in HCAECs co-cultured with HITAECs to those in HCAECs co-cultured with HSaVECs. HIT (Ctrl) and HSV (Ctrl) represent the ratios of transcript levels (measured as DeltaCt) in HCAECs co-cultured with HITAECs or HSaVECs to those in HCAEC monoculture. Results are represented as the heat map; green, gray, and red colours indicate fold change <=0.50, 0.51-1.99, and >=2.00, respectively; ( B ) Western blotting measurements. HCA-HIT represents HCAECs co-cultured with HITAECs, HCA-HSV signifies HCAECs co-cultured with HSaVECs. HCA and HSV signify HCAEC and HSaVEC monocultures, respectively; ( C ) Semi-quantitative analysis of Western blotting results by densitometry. HIT represents the ratio of band density in HCAECs co-cultured with HITAECs to that in HCAECs co-cultured with HSaVECs. HIT (Ctrl) and HSV (Ctrl) abbreviate the ratios of band density in HCAECs co-cultured with HITAECs or HSaVECs to that in HCAEC monoculture. HCA (Ctrl) abbreviates the ratio of band density in HCAEC monoculture to that in HSaVEC monoculture. Snail + Slug are shown at both 56 and 29 kDa values. Results are represented as green, gray, and red colours on t

N-cadherin Antibody in Western Blot (WB) — Figure 9 Proteomic profiling for ECFC, HCAEC and HUVEC. ( A ) Dot blot profiling for 55 angiogenesis-related proteins confirms high similarity between ECFC and mature vascular EC yet also indicates minor differences between these cell lines and generally validating RNA-seq results. Count to the right represents a quantitation of measured angiogenesis-related proteins in terms of their relative expression in HCAEC, HUVEC, and ECFC; ( B ) Western blotting for endothelial phenotype-related markers verifies endothelial identity of PBMC-derived ECFC and demonstrates an intermediate phenotype of PBMC-derived ECFC as compared to HCAEC and HUVEC in terms of HEY2, LYVE1, VEGFR3, Snail and Slug expression. beta-tubulin was probed as a loading control. Count to the bottom represents a quantitation of measured endothelial phenotype-related markers in terms of their relative expression in HCAEC, HUVEC, and ECFC.

N-cadherin Antibody — Antibody specificity was demonstrated by showing that antibodies raised against the same target protein perform similarly. Western blot of N-cadherin using N-cadherin Monoclonal Antibody (Product # MA5-15633), tested in parallel with N-cadherin Monoclonal Antibody (Product # MA1-159), shows similar expression of N-cadherin in the tissue tested. {IAV}

Product Details

MA5-15633

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:200-1:1,000	-
Flow Cytometry (Flow)	1/200 - 1/400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5D5
Immunogen	Purified recombinant fragment of human CDH2 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10985437

Product Specific Information

MA5-15633 targets CDH2 in FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-15633 immunogen is purified recombinant fragment of human CDH2 expressed in E. Coli.

MA5-15633 detects CDH2 which has a predicted molecular weight of approximately 99.8kDa.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Re-expression of circ_0043610 contributes to trophoblast dysfunction through the miR-558/RYBP pathway in preeclampsia.

Endocrine journal

Shang J,Lin L,Huang X,Zhou L,Huang Q

MA5-15633 was used in Western Blotting to investigate the function and the potential mechanism of circ_0043610 in preeclampsia.

Wed Dec 28 00:00:00 EST 2022

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

International journal of molecular sciences

Shishkova DK,Velikanova EA,Bogdanov LA,Sinitsky MY,Kostyunin AE,Tsepokina AV,Gruzdeva OV,Mironov AV,Mukhamadiyarov RA,Glushkova TV,Krivkina EO,Matveeva VG,Hryachkova ON,Markova VE,Dyleva YA,Belik EV,Frolov AV,Shabaev AR,Efimova OS,Popova AN,Malysheva VY,Kolmykov RP,Sevostyanov OG,Russakov DM,Dolganyuk VF,Gutakovsky AK,Zhivodkov YA,Kozhukhov AS,Brusina EB,Ismagilov ZR,Barbarash OL,Yuzhalin AE,Kutikhin AG

MA5-15633 was used in Western Blot to examine the role of calciprotein particles, nanoscale bodies forming in the human blood upon its supersaturation with calcium and phosphate, in cardiovascular disease.

Thu Nov 18 00:00:00 EST 2021

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

International journal of molecular sciences

Shishkova DK,Velikanova EA,Bogdanov LA,Sinitsky MY,Kostyunin AE,Tsepokina AV,Gruzdeva OV,Mironov AV,Mukhamadiyarov RA,Glushkova TV,Krivkina EO,Matveeva VG,Hryachkova ON,Markova VE,Dyleva YA,Belik EV,Frolov AV,Shabaev AR,Efimova OS,Popova AN,Malysheva VY,Kolmykov RP,Sevostyanov OG,Russakov DM,Dolganyuk VF,Gutakovsky AK,Zhivodkov YA,Kozhukhov AS,Brusina EB,Ismagilov ZR,Barbarash OL,Yuzhalin AE,Kutikhin AG

MA5-15633 was used in Western Blot to examine the role of calciprotein particles, nanoscale bodies forming in the human blood upon its supersaturation with calcium and phosphate, in cardiovascular disease.

Thu Nov 18 00:00:00 EST 2021

Calciprotein Particles Cause Endothelial Dysfunction under Flow.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Velikanova E,Bogdanov L,Glushkova T,Kutikhin A

MA5-15633 was used in Western Blotting to study the ability of CPPs to initiate endothelial dysfunction at physiological flow conditions.

Fri Nov 20 00:00:00 EST 2020

Calciprotein Particles Cause Endothelial Dysfunction under Flow.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Velikanova E,Bogdanov L,Glushkova T,Kutikhin A

MA5-15633 was used in Western Blotting to study the ability of CPPs to initiate endothelial dysfunction at physiological flow conditions.

Fri Nov 20 00:00:00 EST 2020

Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Frolov A,Zagorodnikov N,Bogdanov L,Kutikhin A

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Wed Oct 28 00:00:00 EDT 2020

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Cells

Kutikhin AG,Tupikin AE,Matveeva VG,Shishkova DK,Antonova LV,Kabilov MR,Velikanova EA

MA5-15633 was used in Western Blotting to perform a transcriptome-wide study to compare gene expression profiles of endothelial colony-forming cells (ECFC) , human coronary artery endothelial cells (HCAEC), and human umbilical vein endothelial cells (HUVEC).

Fri Apr 03 00:00:00 EDT 2020

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Cells

Kutikhin AG,Tupikin AE,Matveeva VG,Shishkova DK,Antonova LV,Kabilov MR,Velikanova EA

MA5-15633 was used in Western Blotting to perform a transcriptome-wide study to compare gene expression profiles of endothelial colony-forming cells (ECFC) , human coronary artery endothelial cells (HCAEC), and human umbilical vein endothelial cells (HUVEC).

Fri Apr 03 00:00:00 EDT 2020

MicroRNA‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells.

Oncology reports

Alcantara KMM,Garcia RL

MA5-15633 was used in Western Blotting to demonstrate that NF2 is also regulated by microRNAs through direct interaction with evolutionarily conserved microRNA response elements within its 3'-untranslated region.

Mon Apr 01 00:00:00 EDT 2019

Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway.

Molecular medicine reports

Chen F,Zhuang M,Peng J,Wang X,Huang T,Li S,Lin M,Lin H,Xu Y,Li J,Chen Z,Huang Y

MA5-15633 was used in western blot to demonstrate that baicalein inhibits metastasis of cancer cells via inactivation of TGF-beta/Smad4 signaling

Wed Oct 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

Molecular Function: cadherin

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering synapse assembly regulation of signal transduction single organismal cell-cell adhesion regulation of protein localization

Process(es)

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding