Invitrogen

N-cadherin Monoclonal Antibody (5D5)

N-cadherin Antibody in Immunocytochemistry (ICC/IF)

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N-cadherin Antibody (MA5-15633) in ICC/IF

Immunofluorescence analysis of A431 cells using CD325/N Cadherin monoclonal antibody (Product # MA5-15633) (Green). Blue: DRAQ5 fluorescent DNA dye.

N-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

N-cadherin Antibody in Western Blot (WB)

N-cadherin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

MA5-15633

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5D5

Immunogen

Purified recombinant fragment of human CDH2 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10985437

Product Specific Information

MA5-15633 targets CDH2 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-15633 immunogen is purified recombinant fragment of human CDH2 expressed in E. Coli.

MA5-15633 detects CDH2 which has a predicted molecular weight of approximately 99.8kDa.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Re-expression of circ_0043610 contributes to trophoblast dysfunction through the miR-558/RYBP pathway in preeclampsia.

Endocrine journal

Shang J,Lin L,Huang X,Zhou L,Huang Q

MA5-15633 was used in Western Blotting to investigate the function and the potential mechanism of circ_0043610 in preeclampsia.

Wed Dec 28 00:00:00 UTC 2022

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

International journal of molecular sciences

Shishkova DK,Velikanova EA,Bogdanov LA,Sinitsky MY,Kostyunin AE,Tsepokina AV,Gruzdeva OV,Mironov AV,Mukhamadiyarov RA,Glushkova TV,Krivkina EO,Matveeva VG,Hryachkova ON,Markova VE,Dyleva YA,Belik EV,Frolov AV,Shabaev AR,Efimova OS,Popova AN,Malysheva VY,Kolmykov RP,Sevostyanov OG,Russakov DM,Dolganyuk VF,Gutakovsky AK,Zhivodkov YA,Kozhukhov AS,Brusina EB,Ismagilov ZR,Barbarash OL,Yuzhalin AE,Kutikhin AG

MA5-15633 was used in Western Blot to examine the role of calciprotein particles, nanoscale bodies forming in the human blood upon its supersaturation with calcium and phosphate, in cardiovascular disease.

Thu Nov 18 00:00:00 UTC 2021

Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation.

International journal of molecular sciences

Thu Nov 18 00:00:00 UTC 2021

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Cells

Kutikhin AG,Tupikin AE,Matveeva VG,Shishkova DK,Antonova LV,Kabilov MR,Velikanova EA

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Fri Apr 03 00:00:00 UTC 2020

Human Peripheral Blood-Derived Endothelial Colony-Forming Cells Are Highly Similar to Mature Vascular Endothelial Cells yet Demonstrate a Transitional Transcriptomic Signature.

Cells

Kutikhin AG,Tupikin AE,Matveeva VG,Shishkova DK,Antonova LV,Kabilov MR,Velikanova EA

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Fri Apr 03 00:00:00 UTC 2020

Calciprotein Particles Cause Endothelial Dysfunction under Flow.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Velikanova E,Bogdanov L,Glushkova T,Kutikhin A

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Fri Nov 20 00:00:00 UTC 2020

Calciprotein Particles Cause Endothelial Dysfunction under Flow.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Velikanova E,Bogdanov L,Glushkova T,Kutikhin A

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Fri Nov 20 00:00:00 UTC 2020

Co-Culture of Primary Human Coronary Artery and Internal Thoracic Artery Endothelial Cells Results in Mutually Beneficial Paracrine Interactions.

International journal of molecular sciences

Shishkova D,Markova V,Sinitsky M,Tsepokina A,Frolov A,Zagorodnikov N,Bogdanov L,Kutikhin A

Published figure using N-cadherin monoclonal antibody (Product # MA5-15633) in Western Blot

Wed Oct 28 00:00:00 UTC 2020

MicroRNA‑92a promotes cell proliferation, migration and survival by directly targeting the tumor suppressor gene NF2 in colorectal and lung cancer cells.

Oncology reports

Alcantara KMM,Garcia RL

MA5-15633 was used in Western Blotting to demonstrate that NF2 is also regulated by microRNAs through direct interaction with evolutionarily conserved microRNA response elements within its 3'-untranslated region.

Mon Apr 01 00:00:00 UTC 2019

Baicalein inhibits migration and invasion of gastric cancer cells through suppression of the TGF-β signaling pathway.

Molecular medicine reports

Chen F,Zhuang M,Peng J,Wang X,Huang T,Li S,Lin M,Lin H,Xu Y,Li J,Chen Z,Huang Y

MA5-15633 was used in western blot to demonstrate that baicalein inhibits metastasis of cancer cells via inactivation of TGF-beta/Smad4 signaling

Wed Oct 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

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Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD

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UniProt ID: (Human) P19022, (Mouse) P15116, (Rat) Q9Z1Y3

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Entrez Gene ID: (Human) 1000, (Mouse) 12558, (Rat) 83501

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Function(s)

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding enzyme binding metal ion binding cadherin

Process(es)

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering synapse assembly regulation of signal transduction single organismal cell-cell adhesion regulation of protein localization

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N-cadherin Monoclonal Antibody (5D5)

N-cadherin Antibody (MA5-15633) in ICC/IF

Product Details

MA5-15633

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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