OriGene

NAPEPLD Monoclonal Antibody (OTI7B9), TrueMAB™

View all (20) NAPE PLD antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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NAPEPLD Antibody in Immunocytochemistry (ICC/IF) — Anti-NAPEPLD mouse monoclonal antibody (TA503861) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NAPEPLD(RC209877).

NAPEPLD Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-NAPEPLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503861)

NAPEPLD Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAPEPLD (RC209877, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NAPE. Positive lysates LY404703 (100 µg) and LC404703 (20 µg) can be purchased separately from OriGene.

NAPEPLD Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC209877 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NAPEPLD antibody (TA503861), and then analyzed by flow cytometry.

Product Details

CF503861

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI7B9
Immunogen	Full length human recombinant protein of human NAPEPLD produced in HEK293T cell. View immunogen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

N-Acylphosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a membrane-bound enzyme which releases the endocannabinoid anandamide and other bioactive N-acylethanolamines from their corresponding NAPEs in animal tissues. NAPE-PLD has been shown to be expressed by specific populations of neurons in the brain and targeted to axonal processes. Recent studies have suggested that NAEs generated by NAPE-PLD in axons may act as anterograde synaptic signaling molecules that regulate the activity of postsynaptic neurons. NAPE-PLD has also been shown to catalyze the hydrolysis of NAPE to generate OEA (oleoylethanolamide), a natural lipid amide that inhibits food intake in free-feeding rodents by prolonging latency to feed and postmeal interval.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp781D1098; N-acyl phosphatidylethanolamine phospholipase D; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D

Gene Aliases: C7orf18; FMP30; NAPE-PLD; NAPEPLD

UniProt ID: (Human) Q6IQ20

Entrez Gene ID: (Human) 222236

Molecular Function: phospholipase