Invitrogen

NAT8 Monoclonal Antibody (OTI5A8)

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NAT8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of NAT8 in COS7 cells. Cells were transfected with a plasmid overexpressing NAT8 and probed with a NAT8 monoclonal antibody (Product # MA5-24951).

NAT8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a NAT8 monoclonal antibody (Product # MA5-24951) at a dilution of 1:50.

NAT8 Antibody in Western Blot (WB) — Western blot analysis of NAT8 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with NAT8 (Product # MA5-24951) monoclonal antibody.

Product Details

MA5-24951

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5A8
Immunogen	Full-length protein expressed in 293T cell transfected with human NAT8 expression vector View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.77 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724797

Target Information

This protein, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acetyltransferase 2; ATase2; Camello-like protein 1; CCNAT; Cysteinyl-conjugate N-acetyltransferase; kidney- and liver-specific gene product; N-acetyltransferase 8; N-acetyltransferase 8 (camello like); N-acetyltransferase 8 (GCN5-related, putative); NAT8; probable N-acetyltransferase 8

Gene Aliases: ATase2; CCNAT; CML1; GLA; Hcml1; NAT8; TSC501; TSC510

UniProt ID: (Human) Q9UHE5

Entrez Gene ID: (Human) 9027

Molecular Function: acetyltransferase