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Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.5 - 1 µg/mL | View 6 publications 6 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
Assay-dependent | - |
ELISA (ELISA) |
Assay-dependent | - |
ChIP assay (ChIP) |
3 µg/mL | View 1 publication 1 publication |
Gel Shift (GS) |
Assay-dependent | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
- | View 3 publications 3 publications |
Immunohistochemistry - Free Floating (IHC (Free)) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
2A12A7 |
Immunogen |
Recombinant protein (~175 amino acids) from the C-terminal portion of human NF-kappa-B (p65). |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4 |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2533153 |
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene and is present on chromosome 11 in humans. NF-kB P65 is also known as RelA (v-rel avian reticuloendotheliosis viral oncogene homolog A) and belongs to the Rel family of proteins. It is one of the two subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells) that heterodimerizes with the other subunits p50 or p52. However, binding of TNF alpha to its cognate receptor phosphorylates IKK which in turn phosphorylates I kB allowing proteasomal degradation of I kB. It specifically plays a key role in transcription of immunoglobulin k (kappa) gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; MGC131774; NF KB; nf-kappa-b p65; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFkappaB p65; nfkb rela; NFkB-p50; NFKB1; nuclear factor; nuclear factor kappa b; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; REL A; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
Gene Aliases: NFKB3; p65; RELA
UniProt ID: (Human) Q04206, (Mouse) Q04207
Entrez Gene ID: (Human) 5970, (Mouse) 19697
Molecular Function:
Rel homology transcription factor
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