|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein from the N-terminal region of the NR2C subunit of rat GRIN2C|
|Storage buffer||5mM Ammonium bicarbonate, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||3 µl per 200 µg lysate|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
This antibody detects predominantly the NR2C isoform but may show low level cross-reactivity with the NR2A and NR2B isoforms.
In Western blot, this antibody detects a ~140 kDa protein representing NR2C in rat brain homogenate. Faint bands representing the NR2A and NR2B isoforms at 180 KDa may also be observed. Immunohistochemical staining in rat brain with OPA1-04020 yields a pattern consistent with plasma membrane staining.
Reconstitute with 50 ul of PBS.
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Excitatory glycine responses of CNS myelin mediated by NR1/NR3 "NMDA" receptor subunits.
OPA1-04020 was used in western blot to investigate the role of NR1/NR3 NMDA receptor subunits for excitatory glycine responses of CNS myelin
|Piña-Crespo JC,Talantova M,Micu I,States B,Chen HS,Tu S,Nakanishi N,Tong G,Zhang D,Heinemann SF,Zamponi GW,Stys PK,Lipton SA||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:11501)||2010|
Glutamate signaling proteins and tyrosine hydroxylase in the locus coeruleus of alcoholics.
OPA1-04020 was used in western blot to investigate the effect of glutamate signaling proteins and tyrosine hydroxylase on alcoholism
|Karolewicz B,Johnson L,Szebeni K,Stockmeier CA,Ordway GA||Journal of psychiatric research (42:348)||2008|
Elevated levels of the NR2C subunit of the NMDA receptor in the locus coeruleus in depression.
OPA1-04020 was used in western blot to investigate the expression of the NMDA receptor NR2C subunit in the locus coeruleus in major depression patients
|Karolewicz B,Stockmeier CA,Ordway GA||Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (30:1557)||2005|