Proteintech

NUF2 Polyclonal Antibody

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Antibody Testing Data (8)

FIGURE: 1 / 8

NUF2 Antibody (15731-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 15731-1-AP) NUF2 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

Product Image Gallery

NUF2 Antibody in Immunoprecipitation (IP) — IP result of anti-NUF2 (IP:15731-1-AP, 3ug; Detection:15731-1-AP 1:500) with HEK-293 cells lysate 1760ug.

Product Details

15731-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NUF2 Fusion Protein Ag8479 (105-464 aa encoded by BC008489)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: ETADILCPK AKRTSRFLSG IINFIHFREA CRETYMEFLW QYKSSADKMQ QLNAAHQEAL MKLERLDSVP VEEQEEFKQL SDGIQELQQS LNQDFHQKTI VLQEGNSQKK SNISEKTKRL NELKLSVVSL KEIQESLKTK IVDSPEKLKN YKEKMKDTVQ KLKNARQEVV EKYEIYGDSV DCLPSCQLEV QLYQKKIQDL SDNREKLASI LKESLNLEDQ IESDESELKK LKTEENSFKR LMIVKKEKLA TAQFKINKKH EDVKQYKRTV IEDCNKVQEK RGAVYERVTT INQEIQKIKL GIQQLKDAAE REKLKSQEIF LNLKTALEKY HDGIEKAAED SYAKIDEKTA ELKRKMFKMS T (105-464 aa encoded by BC008489)

Target Information

This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation.

International journal of biological sciences

Lin J,Chen X,Yu H,Min S,Chen Y,Li Z,Xie X

Tue Jul 12 00:00:00 EDT 2022

NUF2 Drives Clear Cell Renal Cell Carcinoma by Activating HMGA2 Transcription through KDM2A-mediated H3K36me2 Demethylation.

International journal of biological sciences

Lin J,Chen X,Yu H,Min S,Chen Y,Li Z,Xie X

Tue Jul 12 00:00:00 EDT 2022

NUF2 overexpression contributes to epithelial ovarian cancer progression via ERBB3-mediated PI3K-AKT and MAPK signaling axes.

Frontiers in oncology

Leng R,Meng Y,Sun X,Zhao Y

Wed Jan 11 00:00:00 EST 2023

LncRNA CDKN2B-AS1 stabilized by IGF2BP3 drives the malignancy of renal clear cell carcinoma through epigenetically activating NUF2 transcription.

Cell death & disease

Xie X,Lin J,Fan X,Zhong Y,Chen Y,Liu K,Ren Y,Chen X,Lai D,Li X,Li Z,Tang A

Fri Feb 19 00:00:00 EST 2021

LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores.

Nature communications

Wu M,Chang Y,Hu H,Mu R,Zhang Y,Qin X,Duan X,Li W,Tu H,Zhang W,Wang G,Han Q,Li A,Zhou T,Iwai K,Zhang X,Li H

Thu Jan 17 00:00:00 EST 2019

LUBAC controls chromosome alignment by targeting CENP-E to attached kinetochores.

Nature communications

Wu M,Chang Y,Hu H,Mu R,Zhang Y,Qin X,Duan X,Li W,Tu H,Zhang W,Wang G,Han Q,Li A,Zhou T,Iwai K,Zhang X,Li H

Thu Jan 17 00:00:00 EST 2019

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.

Scientific reports

Shen H,Li L,Teng Z,Meng T,Kong X,Hu Y,Zhu Y,Ma L

Mon Feb 04 00:00:00 EST 2019

Bioinformatics

Protein Aliases: cancer/testis antigen 106; cell division cycle associated 1; Cell division cycle-associated protein 1; hNuf2; hNuf2R; hsNuf2; Kinetochore protein Nuf2; NUF2, NDC80 kinetochore complex component, homolog

Gene Aliases: CDCA1; CT106; NUF2; NUF2R

UniProt ID: (Human) Q9BZD4

Entrez Gene ID: (Human) 83540

Molecular Function: non-motor microtubule binding protein

Function(s)

chromosome segregation sister chromatid cohesion mitotic nuclear division cell division

Process(es)

molecular_function protein binding