Alomone Labs, LTD

Na+/H+ Exchanger 7 (NHE7) (extracellular) Polyclonal Antibody

View all (6) SLC9A7 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of NHE7 in mouse hippocampus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA3 region, showed immunoreactivity of NHE7 (green) in the CA3 pyramidal layer (arrows). B. Pre-incubation of the Antibody with Na+/H+Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007), suppressed staining. Cell nuclei are stained with DAPI (blue).

Na+/H+ Exchanger 7 (NHE7) (extracellular) Antibody in Western Blot (WB) — Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1-2. Anti-Na+/H+Exchanger 7 (NHE7) (extracellular) Antibody (#ANX-007), (1:200). 3-4. Anti-Na+/H+Exchanger 7 (NHE7) (extracellular) Antibody , preincubated with Na+/H+Exchanger 7 (NHE7) (extracellular) Blocking Peptide (BLP-NX007).

Product Details

ANX-007-200UL

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)EISPGKINNVEQND, corresponding to amino acid residues 158-171 of mouse SLC9A7, Extracellular, 2nd loop

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoidmultiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. NHE7 (human 725 aa, chromosome Xp11.4) is ubiquitously expressed, and predominantly localizes to the trans-golgi network. NHE7 mediates the influx of Na+ or K+ in exchange for H+. It is ~70% related to NHE6 but relatively less (~25%) homologous with other NHEs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Na(+)/H(+) exchanger 7; NHE-7; nonselective sodium potassium/proton exchanger; Sodium/hydrogen exchanger 7; solute carrier family 9 (sodium/hydrogen exchanger), isoform 7; solute carrier family 9 (sodium/hydrogen exchanger), member 7; Solute carrier family 9 member 7; solute carrier family 9, subfamily A (NHE7, cation proton antiporter 7), member 7

Gene Aliases: A530087D17Rik; NHE-7; NHE7; SLC9A6; SLC9A7

UniProt ID: (Human) Q96T83, (Mouse) Q8BLV3

Entrez Gene ID: (Human) 84679, (Mouse) 236727, (Rat) 317170

Molecular Function: transporter secondary carrier transporter