Invitrogen

Nanog Monoclonal Antibody (hNanog.2), eBioscience™

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

20 Published Figures

13 References

View all (45) Nanog antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Nanog Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 26

Nanog Antibody (14-5768-82) in ICC/IF

Immunocytochemistry of formaldehyde-fixed and permeabilized N-Tera cells using 5 µg/mL Anti-Human Nanog Purified, followed by 10 µg/mL F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI, colocalization appears pink.

Nanog Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nanog Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Nanog Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Western blot analysis using Nanog Polyclonal antibody (Product # 14-5768-82) showed expression of Nanog in NTERA, NCCIT (Fig. a) and Human iPSC cells (Fig b) but not in HeLa (Fig. a) and lowered Nanog expression in Human derived definitive endoderm (Fig. b) {RE}

Product Details

14-5768-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

Immunocytochemistry (ICC/IF)

5 µg/mL

View 6 publications 6 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

hNanog.2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467572

Product Specific Information

Description: The hNanog.2 antibody recognizes human Nanog, a homeobox transcription factor expressed in embryonic stem cells (ESC). Nanog is essential for self-renewal of ESC and the inner cell mass and is throught to function in the same capacity as other factors such as Oct4 and Sox2. Nanog plays a crucial role in embryonic cell fate specification following formation of the blastocyst by regulating proliferation and preventing differentiation. Nanog functions by directly binding DNA consensus sequences to activate or repress transcription. In differentiating ESC, Nanog binds to its own promoter leading to repression of expression. Nanog has been reported to be overexpressed in germ cell tumors of the gonads and central nervous system as well as breast and colorectal cancer.

Applications Reported: This hNanog.2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This hNanog.2 antibody has been tested by immunoblotting of NTERA cell lysates and can be used at less than or equal to 1 µg/mL. The hNanog.2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high or low pH antigen retrieval and can be used at less than or equal to 20 µg/mL. The hNanog.2 antibody has been tested by immunocytochemistry on formaldehyde fixed and permeabilized cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

BMC developmental biology

Wooldridge LK,Ealy AD

14-5768-82 was used in Immunocytochemistry to explore whether IL6 affects epiblast (EPI) and primitive endoderm (PE) development, the two embryonic lineages generated from the ICM after its formation.

Tue Jan 12 00:00:00 EST 2021

Interleukin-6 promotes primitive endoderm development in bovine blastocysts.

BMC developmental biology

Wooldridge LK,Ealy AD

Tue Jan 12 00:00:00 EST 2021

Generation of an induced pluripotent stem cell line (DANi-011A) from a Parkinson's disease patient with a LRRK2 p.G2019S mutation.

Stem cell research

Chen M,Maimaitili M,Buchholdt SH,Jensen UB,Febbraro F,Denham M

14-5768-82 was used in Immunocytochemistry to show that the expression of pluripotent markers in the iPSC line was confirmed by immunofluorescent staining, and we confirmed its ability to differentiate into the three germ layers.

Fri May 01 00:00:00 EDT 2020

Expression of receptor activator of NFkB (RANK) drives stemness and resistance to therapy in ER+HER2- breast cancer.

Oncotarget

Gomes I,de Almeida BP,Dâmaso S,Mansinho A,Correia I,Henriques S,Cruz-Duarte R,Vilhais G,Félix P,Alves P,Corredeira P,Barbosa-Morais NL,Costa L,Casimiro S

14-5768 was used in Western Blotting to demonstrate that RANK pathway affects the expression of cell cycle regulators and decreases sensitivity to fulvestrant of estrogen receptor (ER)-positive (ER+)/HER2- breast cancer cells, MCF-7 and T47D.

Tue May 12 00:00:00 EDT 2020

Expression of receptor activator of NFkB (RANK) drives stemness and resistance to therapy in ER+HER2- breast cancer.

Oncotarget

Gomes I,de Almeida BP,Dâmaso S,Mansinho A,Correia I,Henriques S,Cruz-Duarte R,Vilhais G,Félix P,Alves P,Corredeira P,Barbosa-Morais NL,Costa L,Casimiro S

Tue May 12 00:00:00 EDT 2020

Initiation of X Chromosome Inactivation during Bovine Embryo Development.

Cells

Yu B,van Tol HTA,Stout TAE,Roelen BAJ

14-5768 was used in Immunocytochemistry-immunoflourescence to confirm that diverse patterns of X-chromosome inactivation (XCI) initiation exist among developing mammalian embryos.

Sun Apr 19 00:00:00 EDT 2020

Initiation of X Chromosome Inactivation during Bovine Embryo Development.

Cells

Yu B,van Tol HTA,Stout TAE,Roelen BAJ

14-5768 was used in Immunocytochemistry-immunoflourescence to confirm that diverse patterns of X-chromosome inactivation (XCI) initiation exist among developing mammalian embryos.

Sun Apr 19 00:00:00 EDT 2020

Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models.

Acta neuropathologica

Shaltouki A,Hsieh CH,Kim MJ,Wang X

14-5768 was used in Immunocytochemistry-immunoflourescence to highlight the importance of mitochondria-associated alpha-synuclein in human disease, and present Miro as a novel therapeutic target.

Mon Oct 01 00:00:00 EDT 2018

Characterization of bovine embryos cultured under conditions appropriate for sustaining human naïve pluripotency.

PloS one

Brinkhof B,van Tol HT,Groot Koerkamp MJ,Wubbolts RW,Haagsman HP,Roelen BA

Thu Sep 07 00:00:00 EDT 2017

NANOG reprograms prostate cancer cells to castration resistance via dynamically repressing and engaging the AR/FOXA1 signaling axis.

Cell discovery

Jeter CR,Liu B,Lu Y,Chao HP,Zhang D,Liu X,Chen X,Li Q,Rycaj K,Calhoun-Davis T,Yan L,Hu Q,Wang J,Shen J,Liu S,Tang DG

Published figure using Nanog monoclonal antibody (Product # 14-5768-82) in Western Blot

Fri Jan 20 00:00:00 EST 2023

NANOG reprograms prostate cancer cells to castration resistance via dynamically repressing and engaging the AR/FOXA1 signaling axis.

Cell discovery

Jeter CR,Liu B,Lu Y,Chao HP,Zhang D,Liu X,Chen X,Li Q,Rycaj K,Calhoun-Davis T,Yan L,Hu Q,Wang J,Shen J,Liu S,Tang DG

Published figure using Nanog monoclonal antibody (Product # 14-5768-82) in Western Blot

Fri Jan 20 00:00:00 EST 2023

Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations.

PloS one

Momcilovic O,Sivapatham R,Oron TR,Meyer M,Mooney S,Rao MS,Zeng X

Fri Jul 07 00:00:00 EDT 2017

Derivation, Characterization, and Neural Differentiation of Integration-Free Induced Pluripotent Stem Cell Lines from Parkinson's Disease Patients Carrying SNCA, LRRK2, PARK2, and GBA Mutations.

PloS one

Momcilovic O,Sivapatham R,Oron TR,Meyer M,Mooney S,Rao MS,Zeng X

Fri Jul 07 00:00:00 EDT 2017

A mRNA landscape of bovine embryos after standard and MAPK-inhibited culture conditions: a comparative analysis.

BMC genomics

Brinkhof B,van Tol HT,Groot Koerkamp MJ,Riemers FM,IJzer SG,Mashayekhi K,Haagsman HP,Roelen BA

14-5768 was used in Immunocytochemistry to characterise the genes involved in bovine pluripotency.

Fri Apr 10 00:00:00 EDT 2015

Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species.

PloS one

Liu B,Badeaux MD,Choy G,Chandra D,Shen I,Jeter CR,Rycaj K,Lee CF,Person MD,Liu C,Chen Y,Shen J,Jung SY,Qin J,Tang DG

14-5768 was used in Immunohistochemistry to reveal unique biochemical properties of Nanog1 in embryonal carcinoma cells and NanogP8 in somatic cancer cells.

Tue Jan 13 00:00:00 EST 2015

Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species.

PloS one

Liu B,Badeaux MD,Choy G,Chandra D,Shen I,Jeter CR,Rycaj K,Lee CF,Person MD,Liu C,Chen Y,Shen J,Jung SY,Qin J,Tang DG

14-5768 was used in Immunohistochemistry to reveal unique biochemical properties of Nanog1 in embryonal carcinoma cells and NanogP8 in somatic cancer cells.

Tue Jan 13 00:00:00 EST 2015

A distinct expression pattern in mammalian testes indicates a conserved role for NANOG in spermatogenesis.

PloS one

Kuijk EW,de Gier J,Lopes SM,Chambers I,van Pelt AM,Colenbrander B,Roelen BA

14-5768 was used in Immunofluorescence to examine NANOG expression in the testes of various mammals, including mouse and human.

Mon Jun 07 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48

View more View less

Gene Aliases: NANOG

View more View less

UniProt ID: (Human) Q9H9S0

View more View less

Entrez Gene ID: (Human) 79923

View more View less

Function(s)

DNA binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding sequence-specific DNA binding homeodomain transcription factor

Process(es)

endodermal cell fate specification transcription, DNA-templated regulation of transcription, DNA-templated cell proliferation regulation of gene expression stem cell population maintenance cell differentiation somatic stem cell population maintenance regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of nucleic acid-templated transcription

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Nanog Monoclonal Antibody (hNanog.2), eBioscience™

Nanog Antibody (14-5768-82) in ICC/IF

Product Details

14-5768-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!