Immunofluorescent analysis of placental Cadherin using Cadherin P Monoclonal Antibody (6A9) (Product# MA1-2003 ) shows staining in BEAS-2B Cells. Cadherin P (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Cadherin P (Product# MA1-2003 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A431 trypsinized membranes.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-2003 detects P-cadherin from human and mouse samples.
MA1-2003 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~120 kDa protein representing P-cadherin from A431 cell extract. MA1-2003 can also be used in immunoprecipitation and immunofluorescence procedures.
The MA1-2003 immunogen is A431 trypsinized membranes.
The cadherin superfamily of proteins are a group of calcium mediated cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Connexins, E-cadherin, Claudin-7 and ß-catenin transiently form junctional nexuses during the post-natal mammary gland development.
MA1-2003 was used in western blot to assess the transiently formed junctional nexuses during post-natal mammary gland development by E-cadherins, connexins, beta-catenin, and claudin-7
|Dianati E,Poiraud J,Weber-Ouellette A,Plante I||Developmental biology (416:52)||2016|
Mechanism of extracellular domain-deleted dominant negative cadherins.
MA1-2003 was used in western blot to investigate the functions of extracellular domain-deleted dominant-negative cadherin in cells
|Nieman MT,Kim JB,Johnson KR,Wheelock MJ||Journal of cell science (112 ( Pt 10):1621)||1999|
|Human||2 ul ascites:ml||
Inhibition of cadherin function differentially affects markers of terminal differentiation in cultured human keratinocytes.
MA1-2003 was used in blocking/activating experiment to investigate the effect of inhibition of cadherin function on keratinocyte differentiation
|Hines MD,Jin HC,Wheelock MJ,Jensen PJ||Journal of cell science (112 ( Pt 24):4569)||1999|