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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
PAK6 Fusion Protein Ag4467 (330-681 aa encoded by BC035596) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.19 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
This antibody has weak cross-reaction with other PAK members.
Immunogen sequence: QKSLRTAPA TGQLPGRSSP AGSPRTWHAQ ISTSNLYLPQ DPTVAKGALA GEDTGVVTHE QFKAALRMVV DQGDPRLLLD SYVKIGEGST GIVCLAREKH SGRQVAVKMM DLRKQQRREL LFNEVVIMRD YQHFNVVEMY KSYLVGEELW VLMEFLQGGA LTDIVSQVRL NEEQIATVCE AVLQALAYLH AQGVIHRDIK SDSILLTLDG RVKLSDFGFC AQISKDVPKR KSLVGTPYWM APEVISRSLY ATEVDIWSLG IMVIEMVDGE PPYFSDSPVQ AMKRLRDSPP PKLKNSHKVS PVLRDFLERM LVRDPQERAT AQELLDHPFL LQTGLPECLV PLIQLYRKQT STC (330-681 aa encoded by BC035596)
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues. Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: p21 (CDKN1A)-activated kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A)-activated kinase 6; p21-activated kinase 6; PAK-5; PAK-6; Serine/threonine-protein kinase PAK 6
Gene Aliases: 4732456M09; PAK5; PAK6
UniProt ID: (Human) Q9NQU5, (Mouse) Q3ULB5
Entrez Gene ID: (Human) 56924, (Mouse) 214230, (Rat) 296078
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