Invitrogen

PAX2 Recombinant Rabbit Monoclonal Antibody (JE25-49)

View all (29) PAX2 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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PAX2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of PAX2 in SKOV-3 cells using a PAX2 monoclonal antibody (Product #MA5-36177). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

PAX2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PAX2 in paraffin-embedded rat epididymis tissue using a monoclonal antibody (Product #MA5-36177). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1:200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

PAX2 Antibody in Western Blot (WB) — Western blot analysis of PAX2 using a PAX2 monoclonal antibody (Product #MA5-36177). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

PAX2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of PAX2 using 293T cells and a PAX2 monoclonal antibody (Product #MA5-36177). The cells were fixed, permeabilized and stained with the primary antibody at a dilution of 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Product Details

MA5-36177

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Expression System	HEK293 cells
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE25-49
Immunogen	Synthetic peptide within Human Pax2 aa 1-50 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2890483

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: optic disc coloboma; paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2

Gene Aliases: FSGS7; Opdc; PAPRS; Pax-2; PAX2

UniProt ID: (Human) Q02962, (Mouse) P32114

Entrez Gene ID: (Human) 5076, (Mouse) 18504, (Rat) 293992

Function(s)

Process(es)

core promoter proximal region sequence-specific DNA binding DNA binding protein binding transcription factor binding superoxide-generating NADPH oxidase activity transcription regulatory region DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding C2H2 zinc finger domain binding sequence-specific DNA binding

PAX2 Recombinant Rabbit Monoclonal Antibody (JE25-49)

Product Details

MA5-36177

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

References (0)

Bioinformatics

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