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PDCD6 Antibody (MA5-44964) in ICC/IF
Immunocytochemistry analysis of PDCD6 in HepG2 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Samples were incubated in PDCD6 Monoclonal antibody (Product # MA5-44964) using a dilution of 1:100 in 2% BSA overnight at 4 followed by Goat Anti-Mouse IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. PBS instead of the primary antibody was used as the secondary antibody only control. Nucl... View More
Product Details
MA5-44964
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A6A7
Immunogen
Recombinant protein within Human PDCD6 aa 1-191/191.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931420
Target Information
An increased intracellular Ca2+ concentration induces apoptotic cell death. Transiently elevated Ca2+ concentrations are required for glucocorticoid-mediated and T cell receptor-mediated pathways, leading to T cell apoptosis. ALG-2 (for apoptosis-linked gene 2) is a Ca2+ -binding protein that participates in regulatory events occuring late in the apoptotic program and where several death signals converge. ALG-2 is a protein expressed in normal brain, and to a greater extent in ischemic brain. The ALG-2 protein contains five EF-hand-like motifs and shares homology with members of the penta EF-hand family, which includes Calpain small subunits sorcin and Grancalcin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALG-2; apoptosis-linked gene 2 protein; Apoptosis-linked gene 2 protein homolog; probable calcium-binding protein ALG-2; Programmed cell death protein 6
Gene Aliases: ALG-2; ALG2; PDCD6; PEF1B
UniProt ID: (Human) O75340
Entrez Gene ID: (Human) 10016
angiogenesis
positive regulation of endothelial cell proliferation
proteolysis
intracellular protein transport
activation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of endothelial cell migration
negative regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of TOR signaling
cellular response to heat
vascular endothelial growth factor receptor-2 signaling pathway
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of angiogenesis
response to calcium ion
negative regulation of protein kinase B signaling
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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