OriGene

PDE4B Monoclonal Antibody (OTI1D12), TrueMAB™

View all (31) PDE4B antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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PDE4B Antibody in Immunocytochemistry (ICC/IF) — Anti-PDE4B mouse monoclonal antibody (TA503471) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B(RC211956).

PDE4B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503471)

PDE4B Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B. Positive lysates LY400919 (100 µg) and LC400919 (20 µg) can be purchased separately from OriGene.

PDE4B Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-PDE4B antibody (TA503471), (Red), compared to a nonspecific negative control antibody, (Blue).

Product Details

TA503471

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI1D12

Immunogen

Full length human recombinant protein of human PDE4B produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

0.62 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cAMP-specific 3',5'-cyclic phosphodiesterase 4B; DKFZp686F2182; DPDE4; dunce-like phosphodiesterase E4; MGC126529; MGC93193; OTTHUMP00000046896; PDE32; phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila); RP5-876C12.1

Gene Aliases: DPDE4; dunce; PDE4B; PDEIVB; R74983

UniProt ID: (Human) Q07343

Entrez Gene ID: (Human) 5142, (Mouse) 18578

Molecular Function: phosphodiesterase