OriGene

PDIA4 Monoclonal Antibody (OTI2C11), TrueMAB™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (44) ERp72 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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PDIA4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-PDIA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503882)

PDIA4 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDIA4 (RC204041, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDIA4. Positive lysates LY401531 (100 µg) and LC401531 (20 µg) can be purchased separately from OriGene.

PDIA4 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in PDIA4 knockout (KO) 293T cell line using Anti-PDIA4 monoclonal antibody (Product # TA503882).

Product Details

TA503882

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Product Specifications

Species Reactivity

Dog, Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

OTI2C11

Immunogen

Full length human recombinant protein of human PDIA4 produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated

Form

liquid

Concentration

0.74 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 5.3.4.1; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; protein disu; protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; Protein disulfide-isomerase A4; Protein ERp-72; testicular tissue protein Li 137

Gene Aliases: ERp-72; ERP70; ERP72; PDIA4

UniProt ID: (Human) P13667

Entrez Gene ID: (Human) 9601, (Dog) 482715

Molecular Function: chaperone