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PHLDA1 Antibody (CF810937) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PHLDA1 (RC220155, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PHLDA. Positive lysates LY402133 (100 µg) and LC402133 (20 µg) can be purchased separately from OriGene.
Product Details
CF810937
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI4D9
Immunogen
Human recombinant protein fragment corresponding to amino acids 183-401 of human PHLDA1 produced in E.coli.
Conjugate
Unconjugated
Form
lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or -transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (APO-1) and its ligand (FAS-L). The human FAS (APO-1) protein is a 48 kDa cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, and is expressed at high levels in T cells subsequent to crosslinking of the T cell receptor (TCR). A previously undescribed protein, TDAG51, restores activation- induced apoptosis in cells that have lost the ability to display Fas in response to activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Apoptosis-associated nuclear protein; Pleckstrin homology-like domain family A member 1; pleckstrin homology-like domain, family A, member 1; PQ-rich protein; PQR protein; Proline- and glutamine-rich protein; Proline- and histidine-rich protein; proline-histidine rich protein; T-cell death associated; T-cell death-associated gene 51 protein
Gene Aliases: DT1P1B11; PHLDA1; PHRIP; Tdag; TDAG51
UniProt ID: (Human) Q8WV24
Entrez Gene ID: (Human) 22822, (Rat) 29380
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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