Invitrogen

PKC epsilon Recombinant Rabbit Monoclonal Antibody (JA38-21)

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PKC epsilon Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of PKC epsilon in Hela cells using a PKC epsilon Monoclonal antibody (Product # MA5-32715) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

PKC epsilon Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of PKC epsilon in 293T cells using a PKC epsilon Monoclonal antibody (Product # MA5-32715) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

PKC epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKC epsilon of paraffin-embedded Human pancreas tissue using a PKC-epsilon Monoclonal antibody (Product #MA5-32715). Counter stained with hematoxylin.

PKC epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKC epsilon of paraffin-embedded Mouse testis tissue using a PKC-epsilon Monoclonal antibody (Product #MA5-32715). Counter stained with hematoxylin.

PKC epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKC epsilon of paraffin-embedded Human kidney tissue using a PKC-epsilon Monoclonal antibody (Product #MA5-32715). Counter stained with hematoxylin.

PKC epsilon Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKC epsilon of paraffin-embedded rat brain tissue using a PKC-epsilon Monoclonal antibody (Product #MA5-32715). Counter stained with hematoxylin.

PKC epsilon Antibody in Western Blot (WB) — Western blot analysis of PKC epsilon in different lysates using a Monoclonal antibody (Product #MA5-32715) at a dilution of 1:1,000. Positive control: Lane1: Jurkat, Lane 2: MCF-7, Lane 3: HT-29.

PKC epsilon Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of PKC epsilon in SH-SY5Y cells using a PKC epsilon Monoclonal Antibody (Product # MA5-32715) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Product Details

MA5-32715

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA38-21

Immunogen

Synthetic peptide within Human PKC epsilon aa 561-610

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809992

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration

Process(es)

actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding