Invitrogen

PKD1 Polyclonal Antibody

Product Image Gallery

PKD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PKD1 in human kidney cancer and adjacent normal tissues. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115779) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

PKD1 Antibody in Western Blot (WB) — Western blot analysis of PKD1 in HeLa cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115779).

Product Details

PA5-115779

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human PKD1(Accession P98161), corresponding to amino acid residues S2454-L2481. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2900414

Target Information

PKD1 gene encodes a member of the polycystin protein family. The encoded glycoprotein contains a large N-terminal extracellular region, multiple transmembrane domains and a cytoplasmic C-tail. It is an integral membrane protein that functions as a regulator of calcium permeable cation channels and intracellular calcium homoeostasis. It is also involved in cell-cell/matrix interactions and may modulate G-protein-coupled signal-transduction pathways. It plays a role in renal tubular development, and mutations in this gene cause autosomal dominant polycystic kidney disease type 1 (ADPKD1). ADPKD1 is characterized by the growth of fluid-filled cysts that replace normal renal tissue and result in end-stage renal failure. Splice variants encoding different isoforms have been noted for this gene. Also, six pseudogenes, closely linked in a known duplicated region on chromosome 16p, have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Autosomal dominant polycystic kidney disease 1 protein; Autosomal dominant polycystic kidney disease 1 protein homolog; PC1; polycystic kidney disease 1 (autosomal dominant); polycystic kidney disease 1 homolog; polycystin-1; polycystic kidney disease-associated protein; polycystin 1; Polycystin-1; transient receptor potential cation channel, subfamily P, member 1

Gene Aliases: mFLJ00285; PBP; Pc-1; PC1; PKD1; TRPP1

UniProt ID: (Human) P98161, (Mouse) O08852

Entrez Gene ID: (Human) 5310, (Rat) 24650, (Mouse) 18763

Molecular Function: ion channel