Invitrogen

PKD1 Polyclonal Antibody

Product Image Gallery

PKD1 Antibody in Western Blot (WB) — Western blot analysis of PKD1 in HeLa cells (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115779).

PKD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PKD1 in mouse liver tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115779) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:200

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human Polycystin 1, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

PKD1 gene encodes a member of the polycystin protein family. The encoded glycoprotein contains a large N-terminal extracellular region, multiple transmembrane domains and a cytoplasmic C-tail. It is an integral membrane protein that functions as a regulator of calcium permeable cation channels and intracellular calcium homoeostasis. It is also involved in cell-cell/matrix interactions and may modulate G-protein-coupled signal-transduction pathways. It plays a role in renal tubular development, and mutations in this gene cause autosomal dominant polycystic kidney disease type 1 (ADPKD1). ADPKD1 is characterized by the growth of fluid-filled cysts that replace normal renal tissue and result in end-stage renal failure. Splice variants encoding different isoforms have been noted for this gene. Also, six pseudogenes, closely linked in a known duplicated region on chromosome 16p, have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Autosomal dominant polycystic kidney disease 1 protein; Autosomal dominant polycystic kidney disease 1 protein homolog; PC1; polycystic kidney disease 1 (autosomal dominant); polycystic kidney disease 1 homolog; polycystin-1; polycystic kidney disease-associated protein; polycystin 1; Polycystin-1; transient receptor potential cation channel, subfamily P, member 1

Gene Aliases: mFLJ00285; PBP; Pc-1; PC1; PKD1; TRPP1

UniProt ID: (Human) P98161, (Mouse) O08852

Entrez Gene ID: (Human) 5310, (Rat) 24650, (Mouse) 18763

Molecular Function: G-protein modulator enzyme modulator ion channel membrane-bound signaling molecule signaling molecule transporter