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PPP3CB Antibody (MA5-50790) in ICC/IF
Immunocytochemistry analysis of PPP3CB in MCF-7 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with PPP3CB recombinant monoclonal antibody (Product # MA5-50790) with a dilution of 1:200 in 2% negative goat serum overnight at 4 . Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibod... View More
Product Details
MA5-50790
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-21
Immunogen
Synthetic peptide within Human Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform aa 265-318/524
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094124
Product Specific Information
Positive control: Mouse brain tissue lysate, Jurkat cell lysate, rat brain tissue lysate, rat skeletal muscle tissue, mouse skeletal muscle tissue, MCF-7.
Predicted band size: 59 kDa
Subcellular Location: Cytoplasm.
Target Information
PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calcineurin A beta; calcineurin A2; Calcineurin subunit A beta; Calmodulin-dependent calcineurin A subunit beta isoform; CAM-PRP catalytic subunit; CaN Abeta; CNA beta; PP2BA beta; protein phosphatase 2B, catalytic subunit, beta isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform; protein phosphatase 3, catalytic subunit, beta isoform; protein phosphatase 3, catalytic subunit, beta isozyme; protein phosphatase from PCR fragment H32; protein phosphatase-2Bb; RP11-345K20.1; Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
Gene Aliases: 1110063J16Rik; CALNA2; CALNB; CNA2; Cnab; CnAbeta; PP2Bbeta; PPP3CB
UniProt ID: (Human) P16298, (Mouse) P48453, (Rat) P20651
Entrez Gene ID: (Human) 5532, (Mouse) 19056, (Rat) 24675
protein serine/threonine phosphatase activity
calcium ion binding
protein binding
calmodulin binding
drug binding
enzyme binding
protein phosphatase 2B binding
calmodulin-dependent protein phosphatase activity
protein dimerization activity
phosphoprotein phosphatase activity
calcium-dependent protein serine/threonine phosphatase activity
hydrolase activity
metal ion binding
protein heterodimerization activity
protein phosphatase
negative regulation of T cell mediated cytotoxicity
lymphangiogenesis
protein phosphorylation
protein dephosphorylation
signal transduction
Wnt signaling pathway, calcium modulating pathway
heart development
learning
memory
dephosphorylation
calcium ion regulated exocytosis
T cell differentiation
locomotion involved in locomotory behavior
calcineurin-NFAT signaling cascade
response to cytokine
social behavior
cellular response to drug
positive regulation of insulin secretion involved in cellular response to glucose stimulus
Fc-epsilon receptor signaling pathway
T cell proliferation
T cell activation
T cell homeostasis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of synaptic plasticity
axon extension
regulation of insulin secretion
positive regulation of NFAT protein import into nucleus
response to stress
regulation of gene expression
response to amphetamine
muscle cell cellular homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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