Keep as concentrated solution.
Positive Control: PC-12, Rat2, MCF-7, MCF-7(500 nM PMA treatment for 1 hr), MCF-7(500 nM PMA treatment for 2 hr), MCF-7(500 nM PMA treatment for 4 hr), MCF-7(500 nM PMA treatment for 6 hr), HUVEC, HUVEC (5 ng/ml VEGF treatment for 10 min), NIH-3T3, NIH-3T3 (starvation for 16 hr and 5 ng/ml PDGF treatment for 15 min).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
The Akt family of serine/threonine protein kinases (also called protein kinase B/PKB) plays a key role in insulin signaling, cellular survival, and transformation. Akt1 is activated by phosphorylation on residues threonine 308 and Serine 473. Thr308, in the catalytic domain, is phosphorylated by the protein kinase PDK1, and Ser473, in the COOH-terminal regulatory domain, is phosphorylated by putative serine 473 kinase. To date, a couple of proteins have been shown to physically interact with PKB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT antibody; AKT1 antibody; AKT1 kinase; AKT1m; Akt1m protein; AKT2 antibody; AKT3 antibody; DKFZp434N0250 antibody; MGC99656 antibody; murine thymoma viral (v-akt) homolog-2 antibody; PKB; PKB alpha; PKB alpha antibody; PKB antibody; PKB beta antibody; PKB gamma antibody; PKB-ALPHA antibody; PKB-GAMMA antibody; PKBB antibody; PKBBETA antibody; PKBG antibody; PRKBA antibody; PRKBB antibody; PRKBG antibody; protein kinase Akt-2 antibody; Protein kinase B; Protein kinase B alpha; protein kinase B alpha antibody; protein kinase B beta antibody; protein kinase B-alpha; Proto-oncogene c-Akt; proto-oncogene c-Akt antibody; RAC antibody; rac protein kinase alpha; rac protein kinase alpha antibody; rac protein kinase beta antibody; RAC-ALPHA antibody; RAC-alpha serine/threonine-protein kinase; RAC-alpha serine/threonine-protein kinase antibody; RAC-BETA antibody; RAC-beta serine/threonine-protein kinase antibody; RAC-gamma antibody; RAC-gamma serine/threonine-protein kinase antibody; RAC-PK-alpha; RAC-PK-alpha antibody; RAC-PK-beta antibody; RAC-PK-gamma antibody; related to A and C kinases; serine-threonine protein kinase; STK-2 antibody; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 1 antibody; v-akt murine thymoma viral oncogene homolog 2 antibody; v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase