This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
This product contains enough material for 10 mini-blots. The immunogenic sequence is conserved among multiple species including human, mouse, and rat in all three isoforms: Akt1, Akt2, and Akt3. Others species (100% homologous) have not been tested. Recommended positive controls include NIH-3T3 cells stimulated with PDGF and 3T3-L1 adipocytes stimulated with OSM and IFN-gamma.
AKT1 is one of 3 closely related ~65 kDa serine/threonine-protein kinases (AKT1, AKT2, and AKT3) called the AKT kinase, also known as Protein Kinase B (PKB) or RAC-alpha, and which plays an important role in diverse biological responses such as regulation of metabolism, cell survival, proliferation/growth, and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates including GSK3beta, caspase 9, BAD, and the Forkhead Transcription Factor. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is activated by PI3K, which in turn can interact with proteins such as FAK (when FAK is phosphorylated at tyrosine 397) thereby linking activation to the cytoskeleton. AKT is phosphorylated on threonine 308 by PDK1 and on serine 473 by PDK2. Phosphorylation at serine 473 is required for full activation of AKT. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at serine 50 negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3alpha at serine 21 and GSK3beta at serine 9, resulting in inhibition of its kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AKT; AKT1 kinase; AKT1m; Akt1m protein; C-AKT; EC 126.96.36.199; kinase Akt1; PKB; PKB alpha; PKB-alpha; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC-alpha serine/threonine kinase; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase