Western blot analysis of Phospho-Aquaporin 2 pSer264 in vasopressin (AVP)-treated rat kidney lysate using a Phospho-Aquaporin 2 pSer264 polyclonal antibody (Product # PA5-35387). Results show a band at ~29kDa and ~37kDa (glycosylated form of the AQP2 protein phosphorylated at Ser264).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 of rat AQP2 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, bovine, chicken, human, mouse and non-human primate based on 100% sequence homology.
PA5-35387 is specific for the ~29kDa AQP2 protein phosphorylated at Ser264, and also recognizes the glycosylated form of AQP2 at ~37kDa. This antibody contains enough material to conduct 10 mini-Western blots.
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.