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Invitrogen
Phospho-BCKDHA (Ser292) Recombinant Rabbit Monoclonal Antibody (BLR060F)
FIGURE: 1 / 1
Phospho-BCKDHA (Ser292) Antibody (MA5-44313) in WB
Western Blot of BCKDHA in whole cell lysate (50 µg) from NIH 3T3, TCMK-1, Hep-G2, Jurkat, LNCaP, GaMG, HEK293T, MCF-7, and differentiated 3T3-L1 cells. Samples were treated with NETN lysis buffer, incubated with BCKDHA monoclonal antibody (Product # MA5-44314) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 30 seconds) Lower Panel: Rabbit anti-RPL10.
Product Details
MA5-44313
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR060F
Immunogen
Surrounding Serine 292
Conjugate
Form
Liquid
Concentration
40 µg/mL
Purification
purified
Storage buffer
BBS, pH 7.8-8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
2-8°C
Shipping conditions
Wet ice
RRID
AB_2926443
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid (BCAA) dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of the branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine. This reaction is the second major step in the catabolism of the branched-chain amino acids (Wynn et al., 1998). The BCKD complex consists of 3 catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a homo-24-meric dihydrolipoyl transacylase (E2), and a homodimeric dihydrolipoamide dehydrogenase (E3). E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains 2 regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKAD E1[a]; BCKDE1A; BCKDH E1-alpha; branched chain keto acid dehydrogenase E1 alpha protein; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Gene Aliases: BCKDE1A; BCKDHA; MSU; MSUD1; OVD1A
UniProt ID: (Human) P12694
Entrez Gene ID: (Human) 593, (Mouse) 12039
alpha-ketoacid dehydrogenase activity
3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity
protein binding
carboxy-lyase activity
metal ion binding
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
protein complex binding
dehydrogenase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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