PA1-060 detects phospho-caveolin-2 Y19 from human and mouse samples.
PA1-060 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~21 kDa protein representing phospho-caveolin-2 Y19 from extract from COS-7 cells transiently transfected with the mouse caveolin-2 gene. Immunofluorescent staining of phospho-caveolin-2 Y19 in NIH 3T3 cells using PA1-060 results in a distinct punctate staining pattern at the cell periphery.
The PA1-060 immunogen is a synthetic phosphopeptide corresponding to residues M(14) A D D A (pY) S H H S G C(25) of mouse CAV2. This peptide (Cat. # PEP-180) is available for use in neutralization and control experiments.
Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: caveolae protein, 20-kD; caveolin 2 isoform a and b; Caveolin-2; Phospho-CAV2
Gene Aliases: AI447843; CAV; CAV2