Western blot analysis of Phospho-HSP27 pSer82 using a Phospho-HSP27 pSer82 polyclonal antibody (Product # PA1-25983). Peptide competition shows that only the peptide corresponding to HSP27 (pSer82) blocks antibody signal, thereby demonstrating the specificity of the antibody.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho peptide (Human)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25983 detects Heat Shock Protein 27 (phospho S82) from human samples. PA1-25983 will not react with endogenous mouse Heat Shock Protein 27 protein phosphorylated on S86 (S86 in mouse is in a region identical to that surrounding S82 in human Heat Shock Protein 27) in extracts of NIH3T3 cells treate
PA1-25983 has been successfully used in Western blot procedures.
The PA1-25983 immunogen is a synthetic phosphopeptide corresponding to a portion of human Heat Shock Protein 27 containing threonine 232.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.