Invitrogen

Phospho-IRE1 alpha (Ser724) Polyclonal Antibody

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Phospho-IRE1 alpha (Ser724) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of IRE1 alpha (Ser724) in immersion-fixed paraffin-embedded sections of human spleen using a polyclonal anti-IRE1 S724 antibody (Product # PA1-16927) at 1:300 for 1 hour at room temperature, followed by incubation with an anti-Rabbit IgG HRP polymer antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the perinuclear cytoplasm in splenocytes.

Phospho-IRE1 alpha (Ser724) Antibody in Western Blot (WB) — Western blot analysis of IRE1 alpha (Ser724) in Hela cells treated (+) or untreated (-) with 10mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 µg/mL anti-IRE1 polyclonal antibody (Product # PA1-16927) in 5% BSA, followed by chemiluminescent detection using an anti-rabbit HRP secondary antibody.

Phospho-IRE1 alpha (Ser724) Antibody — Published figure using Phospho-IRE1 alpha (Ser724) polyclonal antibody (Product # PA1-16927)

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay Dependent

ELISA (ELISA)

1:100-1:2000

Immunocytochemistry (ICC)

1:10-1:500

View 1 publication 1 publication

Immunofluorescence (IF)

1:10-1:500

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

Immunoprecipitation (IP)

1:10-1:500

Western Blot (WB)

1:1000

View 23 publications 23 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Fruit fly, Goat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein.[Swiss-Prot #O75460]

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.025% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2262241

Product Specific Information

Recommended positive controls: Human pancreas whole tissue Lysate (adult whole tumor), Human epididymis whole tissue lysate (adult whole normal)

Target Information

IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase; Serine/threonine-protein kinase/endoribonuclease IRE1

Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P; RGD1559716

UniProt ID: (Human) O75460, (Mouse) Q9EQY0

Entrez Gene ID: (Goat) 102173038, (Human) 2081, (Mouse) 78943, (Rat) 498013, (Rabbit) 100357346

Molecular Function: kinase protein kinase transferase