Invitrogen

Phospho-IRE1 alpha (Ser724) Polyclonal Antibody

Product Image Gallery

Phospho-IRE1 alpha (Ser724) Antibody in Western Blot (WB) — Western blot of Min6 cell lysate showing specific labeling of the ~110 kDa Phospho-IRE1 alpha protein.

Phospho-IRE1 alpha (Ser724) Antibody — Figure 7 Effects of PDI knockdown on ER stress induction and excitatory receptor expressions. ( A ) Western blot data for ER stress makers including pPERK, pIRE1alpha, IRE1alpha and ATF6 at 7 days after PDI siRNA infusion. M, molecular weight marker. ( B ) Western blot data for excitatory receptors including IP3R, NR2A, NR2B, NR1, M1R and CIB1 at 7 days after PDI siRNA infusion. M, molecular weight marker. ( C ) Quantification of effects of PDI siRNA on levels of ER stress markers and receptor expressions (n = 7, respectively). PDI knockdown does not induce ER stress and alterations in excitatory receptor expressions.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:100-1:2000

Immunocytochemistry (ICC)

1:10-1:500

Immunofluorescence (IF)

1:10-1:500

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

Immunoprecipitation (IP)

1:10-1:500

Western Blot (WB)

1:1000

Published Applications

Western Blot (WB)

See 21 publications below

Miscellaneous PubMed (MISC)

See 3 publications below

Immunocytochemistry (ICC)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide surrounding the phosphorylated serine 724 of the human IRE1 alpha protein.[Swiss-Prot #O75460]

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.025% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2262241

Product Specific Information

This antibody does not react with monkey samples.

Suggested positive control: Glucose treated pancreatic islets and endogenous protein of pancreatic beta cells (20 µg).

Target Information

IRE1 alpha is the endoplasmic reticulum to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. IRE1 alpha protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum to nucleus signalling 1; Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; hIRE1p; inositol-requiring 1; inositol-requiring 1 alpha; inositol-requiring enzyme 1; Inositol-requiring protein 1; Inositol-requiring protein 1 antibody; Ire1-alpha; Ire1-alpha anti; IRE1a; IRE1a antibody; IRE1p antibody; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1 antibody

Gene Aliases: 9030414B18Rik; AI225830; C85377; ERN1; hIRE1p; IRE1; IRE1a; Ire1alpha; IRE1P; RGD1559716

UniProt ID: (Human) O75460, (Mouse) Q9EQY0

Entrez Gene ID: (Human) 2081, (Mouse) 78943, (Rat) 498013, (Rabbit) 100357346

Molecular Function: kinase protein kinase transferase