Immunofluorescence analysis of JAK1 [pY1022/1023] was done on 70% confluent log phase COLO 205 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with JAK1[pY1022/1023] Rabbit polyclonal Antibody (44422G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing membrane localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rabbit, Rat, Non-human primate, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JAK1 protein that contains tyrosines 1022 and 1023. The sequence is conserved in mouse and pig.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
ALK-activating homologous mutations in LTK induce cellular transformation.
44-422G was used in western blot to examine the transforming potential of leukocyte tyrosine kinase
|Roll JD,Reuther GW||PloS one (7:null)||2012|
IL-6 promotes head and neck tumor metastasis by inducing epithelial-mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway.
44-422G was used in western blot to determine the function of IL-6 in the epithelial-mesenchymal transition in head and neck tumor cells.
|Yadav A,Kumar B,Datta J,Teknos TN,Kumar P||Molecular cancer research : MCR (9:1658)||2011|
SOCS-1 binding to tyrosine 441 of IFN-gamma receptor subunit 1 contributes to the attenuation of IFN-gamma signaling in vivo.
44-422G was used in western blot to elucidate how suppressor of cytokine signaling-1 regulates IFN-gamma receptor subunit 1 using a new mouse model.
|Starr R,Fuchsberger M,Lau LS,Uldrich AP,Goradia A,Willson TA,Verhagen AM,Alexander WS,Smyth MJ||Journal of immunology (Baltimore, Md. : 1950) (183:4537)||2009|
|Not Applicable||Not Cited||
'Tuning' of type I interferon-induced Jak-STAT1 signaling by calcium-dependent kinases in macrophages.
44-422G was used in western blot to study the function of CaMK and Pyk2 downstream of ITAM-associated receptors in the regulation of cytokine-induced activation of Jak kinases and STAT transcription factors
|Wang L,Tassiulas I,Park-Min KH,Reid AC,Gil-Henn H,Schlessinger J,Baron R,Zhang JJ,Ivashkiv LB||Nature immunology (9:186)||2008|
|Human||Not Cited||Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.||Ratthé C,Pelletier M,Chiasson S,Girard D||Journal of leukocyte biology (81:1287)||2007|
|Rabbit||Not Cited||Erythropoietin protects the infant heart against ischemia-reperfusion injury by triggering multiple signaling pathways.||Rafiee P,Shi Y,Su J,Pritchard KA,Tweddell JS,Baker JE||Basic research in cardiology (100:187)||2005|
|Non-human primate||Not Cited||Mumps virus V protein antagonizes interferon without the complete degradation of STAT1.||Kubota T,Yokosawa N,Yokota S,Fujii N,Tashiro M,Kato A||Journal of virology (79:4451)||2005|
|Mouse||Not Cited||Role of tyrosine 441 of interferon-gamma receptor subunit 1 in SOCS-1-mediated attenuation of STAT1 activation.||Qing Y,Costa-Pereira AP,Watling D,Stark GR||The Journal of biological chemistry (280:1849)||2005|
|Rat||Not Cited||Falcarindiol impairs the expression of inducible nitric oxide synthase by abrogating the activation of IKK and JAK in rat primary astrocytes.||Shiao YJ,Lin YL,Sun YH,Chi CW,Chen CF,Wang CN||British journal of pharmacology (144:42)||2005|
|Human||Not Cited||Growth arrest of epithelial cells during measles virus infection is caused by upregulation of interferon regulatory factor 1.||Yokota S,Okabayashi T,Yokosawa N,Fujii N||Journal of virology (78:4591)||2004|
|Human||Not Cited||Functional expression of the interleukin-11 receptor alpha-chain and evidence of antiapoptotic effects in human colonic epithelial cells.||Kiessling S,Muller-Newen G,Leeb SN,Hausmann M,Rath HC,Strater J,Spottl T,Schlottmann K,Grossmann J,Montero-Julian FA,Scholmerich J,Andus T,Buschauer A,Heinrich PC,Rogler G||The Journal of biological chemistry (279:10304)||2004|
|Human||Not Cited||C-terminal region of STAT-1alpha is not necessary for its ubiquitination and degradation caused by mumps virus V protein.||Yokosawa N,Yokota S,Kubota T,Fujii N||Journal of virology (76:12683)||2002|
IL-2 induces conformational changes in its preassembled receptor core, which then migrates in lipid raft and binds to the cytoskeleton meshwork.
44-422G was used in western blot to study the assembly of full-length IL-2R at the surface of living lymphocytes
|Pillet AH,Lavergne V,Pasquier V,Gesbert F,Thèze J,Rose T||Journal of molecular biology (403:671)||2010|
Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
44-422G was used in western blot to elucidate IL-7 signaling in human T cells
|Rose T,Pillet AH,Lavergne V,Tamarit B,Lenormand P,Rousselle JC,Namane A,Thèze J||The Journal of biological chemistry (285:14898)||2010|
||Mumps virus V protein antagonizes interferon without the complete degradation of STAT1.||Kubota T,Yokosawa N,Yokota S,Fujii N,Tashiro M,Kato A||Journal of virology (79:4451)||2005|