Western blot analysis of Phospho-NMDA Receptor 2A pTyr1325 in rat hippocampal lysate using a Phospho-NMDA Receptor 2A pTyr1325 polyclonal antibody (Product # PA5-35400). Results show a band at ~180kDa. The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide used as antigen but not by the dephosphopeptide (not shown).
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 of rat GRIN2A conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.1mg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody contains enough material to conduct 10 mini-Western blots.
The NMDA Receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. The NMDAR is also potentiated by protein phosphorylation. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of th NMDAR2 (NR2 A-D) subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.