Western blot analysis of Phospho-OLIG2 pSer10+pSer13+pSer14 in rat neonatal brain lysate using a Phospho-OLIG2 pSer10+pSer13+pSer14 polyclonal antibody (Product # PA5-35405). Results show a band at ~32kDa. Immunolabeling is blocked by the phospho-peptide used as antigen (lane 2), but not by the corresponding dephospho-peptide (not shown).
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 of human Olig2 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 50% glycerol, 0.1mg/ml BSA, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with guinea pig, mouse, human, non-human primate and zebrafish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
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