|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human PED/PEA-15 containing serine 116.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26007 detects PEA15 (phospho S116) from human and mouse samples. PA1-26007 is expected to cross react with rat (100% conserved) due to sequence homology.
PA1-26007 has been successfully used in Western blot procedures. For positive control, use Cos cetss transfected with PEDcDNA and stimulated with 100µg/ml PMA; SK-BR-3 cells.
The PA1-26007 immunogen is a synthetic phosphopeptide corresponding to a portion of human PED/PEA-15 containing serine 116.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.