Invitrogen

Phospho-SGTA (Ser301) Polyclonal Antibody

Product Image Gallery

Phospho-SGTA (Ser301) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-SGTA (Ser301) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-SGTA (Ser301) polyclonal antibody (Product # PA5-105402) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Phospho-SGTA (Ser301) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-SGTA (Ser301) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-SGTA (Ser301) polyclonal antibody (Product # PA5-105402) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-SGTA (Ser301) Antibody in Western Blot (WB) — Western blot analysis of Phospho-SGTA (Ser301) in mouse liver tissue (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-SGTA (Ser301) polyclonal antibody (Product # PA5-105402).

Product Details

PA5-105402

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human SGTA around the phosphorylation site of Ser301. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	sequential chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2816830

Target Information

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: alpha h UBP; Alpha-SGT; protein containing three tetratricopeptide repeats; Small glutamine-rich protein with tetratricopeptide repeats 1; small glutamine-rich tetratricopeptide repeat (TPR) containing protein; small glutamine-rich tetratricopeptide repeat (TPR) containing protein (SGT); small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha; Small glutamine-rich tetratricopeptide repeat-containing protein alpha; UBP; Vpu-binding protein

Gene Aliases: 5330427H01Rik; AI194281; alphaSGT; D10Ertd190e; hSGT; SGT; SGT1; SGTA; Stg

UniProt ID: (Human) O43765, (Mouse) Q8BJU0, (Rat) O70593

Entrez Gene ID: (Human) 6449, (Mouse) 52551, (Rat) 64667

Function(s)

biological_process viral process positive regulation of ER-associated ubiquitin-dependent protein catabolic process

Process(es)

protein binding BAT3 complex binding protein homodimerization activity protein heterodimerization activity