Peptide Competition. Lysates prepared from NIH3T3 cells left untreated (1) or treated with PDGF (2-5). were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% BSA-TBST buffer for one hour at room temperature, and incubated with SHP2 [pY580] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab and quote;)2 anti-rabbit IgG HRP conjugate (Cat. no. ALI4404) and bands were detected using the Pierce SuperSignal™ method. The data show that only the peptide corresponding to SHP2 [pY580] blocks the signal, verifying the specificity of the antibody. This antibody was found not to cross-react with SHP2 phosphorylated on tyrosines 304, 327 or 542 (data not shown).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains tyrosine 580. The sequence is conserved in mouse, rat and chicken.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. infection.
44-558G was used to identify host signaling dynamics upon Burkholderia spp. infection by a reverse-phase protein microarray-based screen
|Chiang CY,Uzoma I,Lane DJ,Memi¿evi¿ V,Alem F,Yao K,Kota KP,Bavari S,Wallqvist A,Hakami RM,Panchal RG||Frontiers in microbiology (6:null)||2015|