Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 4
Phospho-TRAP220 (Thr1457) Antibody (PA5-104998) in ICC/IF
Immunofluorescent analysis of Phospho-TRAP220 (Thr1457) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-TRAP220 (Thr1457) polyclonal antibody (Product # PA5-104998) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa F... View More
Product Details
PA5-104998
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MED1(Accession Q15648), corresponding to amino acid residues around phosphorylated Thr1457.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816471
Product Specific Information
Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.
Target Information
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; peroxisome proliferator activated receptor binding protein; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARG binding protein; PPRB; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205
Gene Aliases: AI480703; ARC205; CRSP1; CRSP200; CRSP210; DRIP205; DRIP230; l11Jus15; MED1; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
UniProt ID: (Human) Q15648, (Mouse) Q925J9
Entrez Gene ID: (Human) 5469, (Mouse) 19014
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter binding
RNA polymerase II transcription cofactor activity
chromatin binding
transcription cofactor activity
transcription coactivator activity
receptor activity
protein binding
transcription factor binding
ligand-dependent nuclear receptor binding
estrogen receptor binding
ligand-dependent nuclear receptor transcription coactivator activity
thyroid hormone receptor coactivator activity
chromatin DNA binding
nuclear hormone receptor binding
mediator complex binding
vitamin D receptor binding
retinoic acid receptor binding
peroxisome proliferator activated receptor binding
thyroid hormone receptor binding
LBD domain binding
ubiquitin protein ligase activity
DNA binding
protein complex binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
angiogenesis
liver development
embryonic placenta development
lens development in camera-type eye
thyroid hormone mediated signaling pathway
ventricular trabecula myocardium morphogenesis
retinal pigment epithelium development
regulation of transcription from RNA polymerase I promoter
transcription initiation from RNA polymerase II promoter
thyroid hormone generation
androgen biosynthetic process
brain development
lactation
positive regulation of gene expression
negative regulation of keratinocyte proliferation
protein ubiquitination
keratinocyte differentiation
monocyte differentiation
intracellular steroid hormone receptor signaling pathway
androgen receptor signaling pathway
organ regeneration
positive regulation of intracellular estrogen receptor signaling pathway
positive regulation of protein import into nucleus, translocation
positive regulation of mammary gland epithelial cell proliferation
embryonic heart tube development
embryonic hindlimb morphogenesis
embryonic hemopoiesis
peroxisome proliferator activated receptor signaling pathway
cellular response to hepatocyte growth factor stimulus
megakaryocyte development
mRNA transcription from RNA polymerase II promoter
negative regulation of apoptotic process
cellular lipid metabolic process
fat cell differentiation
positive regulation of keratinocyte differentiation
positive regulation of erythrocyte differentiation
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
erythrocyte development
enucleate erythrocyte development
regulation of cell cycle
positive regulation of interferon-gamma-mediated signaling pathway
mammary gland branching involved in thelarche
mammary gland branching involved in pregnancy
epithelial cell proliferation involved in mammary gland duct elongation
positive regulation of G0 to G1 transition
ERK1 and ERK2 cascade
regulation of vitamin D receptor signaling pathway
cellular response to epidermal growth factor stimulus
cellular response to steroid hormone stimulus
cellular response to thyroid hormone stimulus
positive regulation of receptor activity
positive regulation of hepatocyte proliferation
regulation of RNA biosynthetic process
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
multicellular organism development
heart development
positive regulation of cell proliferation
organ morphogenesis
mammary gland epithelial cell proliferation
camera-type eye development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support